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Article

The Janus Role of Adhesion in Chondrogenesis

1
Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain
2
Department of Electronics and Biomedical Engineering, University of Barcelona (UB), 08028 Barcelona, Spain
3
Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain
4
Departamento de Química Orgánica, Universidad de Málaga-IBIMA, 29071 Málaga, Spain
5
Centro Andaluz de Nanomedicina y Biotecnología-BIONAND, Campanillas, 29590 Málaga, Spain
6
Department of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, 29071 Málaga, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(15), 5269; https://doi.org/10.3390/ijms21155269
Received: 25 May 2020 / Revised: 23 July 2020 / Accepted: 23 July 2020 / Published: 24 July 2020
(This article belongs to the Special Issue Dual Function Molecules and Processes in Cell Fate Decision)
Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell–cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell–substrate adhesion in the tissue engineering strategies for cartilage repair. View Full-Text
Keywords: dendrimer; nanopatterning; RGD; mesenchymal cell condensation; cell–cell interactions; YAP; chondrogenesis dendrimer; nanopatterning; RGD; mesenchymal cell condensation; cell–cell interactions; YAP; chondrogenesis
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MDPI and ACS Style

Casanellas, I.; Lagunas, A.; Vida, Y.; Pérez-Inestrosa, E.; Andrades, J.A.; Becerra, J.; Samitier, J. The Janus Role of Adhesion in Chondrogenesis. Int. J. Mol. Sci. 2020, 21, 5269. https://doi.org/10.3390/ijms21155269

AMA Style

Casanellas I, Lagunas A, Vida Y, Pérez-Inestrosa E, Andrades JA, Becerra J, Samitier J. The Janus Role of Adhesion in Chondrogenesis. International Journal of Molecular Sciences. 2020; 21(15):5269. https://doi.org/10.3390/ijms21155269

Chicago/Turabian Style

Casanellas, Ignasi, Anna Lagunas, Yolanda Vida, Ezequiel Pérez-Inestrosa, José A. Andrades, José Becerra, and Josep Samitier. 2020. "The Janus Role of Adhesion in Chondrogenesis" International Journal of Molecular Sciences 21, no. 15: 5269. https://doi.org/10.3390/ijms21155269

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