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Article

3-D Nucleus Architecture in Oat × Maize Addition Lines

1
Institute of Biology, Biotechnology, and Environmental Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland
2
Department of Plant Breeding, Physiology, and Seed Science, University of Agriculture in Kraków, Podłużna 3, 30-239 Kraków, Poland
3
Department of Biotechnology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(12), 4280; https://doi.org/10.3390/ijms21124280
Received: 30 May 2020 / Revised: 10 June 2020 / Accepted: 12 June 2020 / Published: 16 June 2020
(This article belongs to the Special Issue Plant Cell and Organism Development)
The nucleus architecture of hybrid crop plants is not a well-researched topic, yet it can have important implications for their genetic stability and usefulness in the successful expression of agronomically desired traits. In this work we studied the spatial distribution of introgressed maize chromatin in oat × maize addition lines with the number of added maize chromosomes varying from one to four. The number of chromosome additions was confirmed by genomic in situ hybridization (GISH). Maize chromosome-specific simple sequence repeat (SSR) markers were used to identify the added chromosomes. GISH on 3-D root and leaf nuclei was performed to assess the number, volume, and position of the maize-chromatin occupied regions. We revealed that the maize chromosome territory (CT) associations of varying degree prevailed in the double disomic lines, while CT separation was the most common distribution pattern in the double monosomic line. In all analyzed lines, the regions occupied by maize CTs were located preferentially at the nuclear periphery. A comparison between the tissues showed that the maize CTs in the leaf nuclei are positioned closer to the center of the nucleus than in the root nuclei. These findings shed more light on the processes that shape the nucleus architecture in hybrids. View Full-Text
Keywords: addition line; Avena sativa L.; chromosome territory; GISH; hybrid; nucleus; Zea mays L. addition line; Avena sativa L.; chromosome territory; GISH; hybrid; nucleus; Zea mays L.
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MDPI and ACS Style

Idziak-Helmcke, D.; Warzecha, T.; Sowa, M.; Warchoł, M.; Dziurka, K.; Czyczyło-Mysza, I.; Skrzypek, E. 3-D Nucleus Architecture in Oat × Maize Addition Lines. Int. J. Mol. Sci. 2020, 21, 4280. https://doi.org/10.3390/ijms21124280

AMA Style

Idziak-Helmcke D, Warzecha T, Sowa M, Warchoł M, Dziurka K, Czyczyło-Mysza I, Skrzypek E. 3-D Nucleus Architecture in Oat × Maize Addition Lines. International Journal of Molecular Sciences. 2020; 21(12):4280. https://doi.org/10.3390/ijms21124280

Chicago/Turabian Style

Idziak-Helmcke, Dominika, Tomasz Warzecha, Marta Sowa, Marzena Warchoł, Kinga Dziurka, Ilona Czyczyło-Mysza, and Edyta Skrzypek. 2020. "3-D Nucleus Architecture in Oat × Maize Addition Lines" International Journal of Molecular Sciences 21, no. 12: 4280. https://doi.org/10.3390/ijms21124280

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