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Article

Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved Arabidopsis

1
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China
2
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, China
3
College of Life Sciences, Northeast Forestry University, Harbin 150040, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(11), 4027; https://doi.org/10.3390/ijms21114027
Received: 5 May 2020 / Revised: 29 May 2020 / Accepted: 2 June 2020 / Published: 4 June 2020
Nitrogen (N) is an essential macronutrient for plant growth. Plants absorb and utilize N mainly in the form of nitrate (NO3) or ammonium (NH4+). In this study, the nitrate transporter DsNRT3.1 (also known as the nitrate assimilation-related protein DsNAR2.1) was characterized from Dianthus spiculifolius. A quantitative PCR (qPCR) analysis showed that the DsNRT3.1 expression was induced by NO3. Under N-starvation conditions, the transformed Arabidopsis seedlings expressing DsNRT3.1 had longer roots and a greater fresh weight than the wild type. Subcellular localization showed that DsNRT3.1 was mainly localized to the plasma membrane in Arabidopsis root hair cells. Non-invasive micro-test (NMT) monitoring showed that the root hairs of N-starved transformed Arabidopsis seedlings had a stronger NO3 and NH4+ influx than the wild-type seedlings, using with NO3 or NH4+ as the sole N source; contrastingly, transformed seedlings only had a stronger NO3 influx when NO3 and NH4+ were present simultaneously. In addition, the qPCR analysis showed that the expression of AtNRT2 genes (AtNRT2.1–2.6), and particularly of AtNRT2.5, in the transformed Arabidopsis differed from that in the wild type. Overall, our results suggest that the heterologous expression of DsNRT3.1 affects seedlings’ growth by enhancing the NO3 and NH4+ uptake in N-starved Arabidopsis. This may be related to the differential expression of AtNRT2 genes. View Full-Text
Keywords: nitrogen; DsNRT3.1; heterologous expression; NO3 and NH4+ uptake; seedling growth; Arabidopsis nitrogen; DsNRT3.1; heterologous expression; NO3 and NH4+ uptake; seedling growth; Arabidopsis
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MDPI and ACS Style

Ma, H.; Zhao, J.; Feng, S.; Qiao, K.; Gong, S.; Wang, J.; Zhou, A. Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved Arabidopsis. Int. J. Mol. Sci. 2020, 21, 4027. https://doi.org/10.3390/ijms21114027

AMA Style

Ma H, Zhao J, Feng S, Qiao K, Gong S, Wang J, Zhou A. Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved Arabidopsis. International Journal of Molecular Sciences. 2020; 21(11):4027. https://doi.org/10.3390/ijms21114027

Chicago/Turabian Style

Ma, Hongping, Junchao Zhao, Shuang Feng, Kun Qiao, Shufang Gong, Jingang Wang, and Aimin Zhou. 2020. "Heterologous Expression of Nitrate Assimilation Related-Protein DsNAR2.1/NRT3.1 Affects Uptake of Nitrate and Ammonium in Nitrogen-Starved Arabidopsis" International Journal of Molecular Sciences 21, no. 11: 4027. https://doi.org/10.3390/ijms21114027

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