1. Introduction
2. Results
2.1. Ticagrelor Attenuates polyP-Induced NETs without Affecting polyP Secretion from Platelets
2.2. Ticagrelor Effect on Neutrophils Does not Rely on P2Y12 Receptor and Autophagy
2.3. Ticagrelor Has no Effect on Intracellular Expression of TF
2.4. Ticagrelor Inhibits Thrombotic NET Release Induced by Drug Eluting Coronary Stents
3. Discussion
4. Material and Methods
4.1. Patients and Sample Collection
4.2. Stimulation and Inhibition Studies
4.3. PolyP Detection Using Fluorescent Probes
4.4. Other Experimental Procedures
4.4.1. Immunofluorescence Staining
4.4.2. NET Structures Isolation
4.4.3. MPO/DNA Complex ELISA
4.4.4. Flow Cytometry Analysis
4.4.5. RNA Isolation, cDNA Synthesis, and qRT-PCR
4.4.6. Western Blot Analysis
4.5. Statistical Analysis
4.6. Ethical Approval
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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