Next Article in Journal
Biocatalytic Amplification of UV Signal in Capillary Electrophoresis of MicroRNA
Previous Article in Journal
Missing Link Between Molecular Aspects of Ventricular Arrhythmias and QRS Complex Morphology in Left Ventricular Hypertrophy
Previous Article in Special Issue
“Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
Open AccessArticle

Improving Phenolic Total Content and Monoterpene in Mentha x piperita by Using Salicylic Acid or Methyl Jasmonate Combined with Rhizobacteria Inoculation

1
INBIAS (CONICET-Universidad Nacional de Río Cuarto), Campus Universitario, 5800 Río Cuarto, Argentina
2
Department of Biochemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745 Jena, Germany
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 50; https://doi.org/10.3390/ijms21010050
Received: 9 November 2019 / Revised: 13 December 2019 / Accepted: 16 December 2019 / Published: 19 December 2019
(This article belongs to the Special Issue Salicylic Acid Signalling in Plants)
The effects of plant inoculation with plant growth-promoting rhizobacteria (PGPR) and those resulting from the exogenous application of salicylic acid (SA) or methyl jasmonte (MeJA) on total phenolic content (TPC) and monoterpenes in Mentha x piperita plants were investigated. Although the PGPR inoculation response has been studied for many plant species, the combination of PGPR and exogenous phytohormones has not been investigated in aromatic plant species. The exogenous application of SA produced an increase in TPC that, in general, was of a similar level when applied alone as when combined with PGPR. This increase in TPC was correlated with an increase in the activity of the enzyme phenylalanine ammonia lyase (PAL). Also, the application of MeJA at different concentrations in combination with inoculation with PGPR produced an increase in TPC, which was more relevant at 4 mM, with a synergism effect being observed. With respect to the main monoterpene concentrations present in peppermint essential oil (EO), it was observed that SA or MeJA application produced a significant increase similar to that of the combination with rhizobacteria. However, when plants were exposed to 2 mM MeJA and inoculated, an important increase was produced in the concentration on menthol, pulegone, linalool, limonene, and menthone concentrations. Rhizobacteria inoculation, the treatment with SA and MeJA, and the combination of both were found to affect the amount of the main monoterpenes present in the EO of M. piperita. For this reason, the expressions of genes related to the biosynthesis of monoterpene were evaluated, with this expression being positively affected by MeJA application and PGPR inoculation, but was not modified by SA application. Our results demonstrate that MeJA or SA application combined with inoculation with PGPR constitutes an advantageous management practice for improving the production of secondary metabolites from M. piperita. View Full-Text
Keywords: salicylic acid; jasmonic acid; rhizobacteria; plant growth-promoting rhizobacteria (PGPR); mint; total phenolic content; monoterpene; menthol; pulegone salicylic acid; jasmonic acid; rhizobacteria; plant growth-promoting rhizobacteria (PGPR); mint; total phenolic content; monoterpene; menthol; pulegone
Show Figures

Figure 1

MDPI and ACS Style

Cappellari, L.R.; Santoro, M.V.; Schmidt, A.; Gershenzon, J.; Banchio, E. Improving Phenolic Total Content and Monoterpene in Mentha x piperita by Using Salicylic Acid or Methyl Jasmonate Combined with Rhizobacteria Inoculation. Int. J. Mol. Sci. 2020, 21, 50.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop