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Article

Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation

by
Na-Hyun Shin
1,†,
Do Thi Trang
2,†,
Woo-Jong Hong
3,†,
Kiyoon Kang
4,
Jadamba Chuluuntsetseg
2,
Joon-Kwan Moon
2,
Yo-Han Yoo
3,
Ki-Hong Jung
3,* and
Soo-Cheul Yoo
2,*
1
Graduate School of Integrated Bioindustry, Sejong University, Seoul 05006, Korea
2
Department of Plant Life and Environmental Science, Hankyong National University, 327, Jungangro, Anseong-si, Gyeonggi-do 17579, Korea
3
Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Korea
4
Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(1), 260; https://doi.org/10.3390/ijms21010260
Submission received: 26 November 2019 / Revised: 27 December 2019 / Accepted: 28 December 2019 / Published: 30 December 2019
(This article belongs to the Collection Feature Papers in Molecular Plant Sciences)
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Abstract

:
Chlorophyll breakdown is a vital catabolic process of leaf senescence as it allows the recycling of nitrogen and other nutrients. In the present study, we isolated rice senescence-induced receptor-like kinase (OsSRLK), whose transcription was upregulated in senescing rice leaves. The detached leaves of ossrlk mutant (ossrlk) contained more green pigment than those of the wild type (WT) during dark-induced senescence (DIS). HPLC and immunoblot assay revealed that degradation of chlorophyll and photosystem II proteins was repressed in ossrlk during DIS. Furthermore, ultrastructural analysis revealed that ossrlk leaves maintained the chloroplast structure with intact grana stacks during dark incubation; however, the retained green color and preserved chloroplast structures of ossrlk did not enhance the photosynthetic competence during age-dependent senescence in autumn. In ossrlk, the panicles per plant was increased and the spikelets per panicle were reduced, resulting in similar grain productivity between WT and ossrlk. By transcriptome analysis using RNA sequencing, genes related to phytohormone, senescence, and chlorophyll biogenesis were significantly altered in ossrlk compared to those in WT during DIS. Collectively, our findings indicate that OsSRLK may degrade chlorophyll by participating in a phytohormone-mediated pathway.

1. Introduction

Chlorophyll plays a pivotal role in photosynthesis by absorbing light and transferring the light energy and electrons to other molecules [1]. Two types of chlorophylls exist, namely, chlorophyll a (Chl a) and chlorophyll b (Chl b). While Chl a serves as a component of all chlorophyll–protein complexes, such as photosystem I (PSI), photosystem II (PSII), and cytochrome b6f complex, Chl b is found only in the PSI-associated light-harvesting complex I (LHCI) and PSII-associated LHCII, whose apoproteins are encoded by the Lhca and Lhcb gene families, respectively [2]. LHCI is located in the stroma lamellae of the thylakoid membrane, whereas LHCII is mainly present in the grana, the stacking region of the thylakoid membranes, and its intermolecular interaction is required for the formation and maintenance of grana stacks [3,4].
Chlorophyll degradation is the visual symptom of leaf senescence, which is accompanied by the breakdown of chlorophyll–protein complexes and thylakoid membranes [5]. This irreversible process occurs via sequential reactions catalyzed by the chlorophyll-degrading enzymes at LHCII [6,7]. Initially, Chl b is converted to Chl a by Chl b reductase, which is encoded by non-yellow coloring 1 (NYC1) and NYC1-like (NOL), and by 7-hydroxymethyl Chl a reductase (HCAR) [2,8,9,10]. The removal of magnesium (Mg) from Chl a is catalyzed by Mg-dechelatase, encoded by Mendel’s green cotyledon genes (Non-yellowing/Stay-green (NYE/SGR) genes) to produce pheophytin a (Phein a) [11,12]. Phein a is then converted to pheophorbide a (Pheide a) by pheophytin pheophorbide hydrolase (PPH) [13]. The porphyrin ring of Pheide a is cleaved by pheophorbide a oxygenase (PAO), producing red Chl catabolite (RCC) and thus losing its green color [14,15]. Furthermore, RCC is catalyzed by red Chl catabolite reductase (RCCR) to produce primary fluorescent Chl catabolite (pFCC) [16]. This pFCC is then transported into vacuoles and isomerized to nonfluorescent Chl catabolite (NCC) [17].
Leaf senescence generally occurs in an age-dependent manner; however, it can be affected by internal and environmental factors, such as phytohormones, pathogen infection, extreme temperatures, salt, drought, nutrient deficiency, and shading [18,19,20]. Thus, genetic regulators controlling phytohormone biosynthesis and signaling affect the onset and progress of leaf senescence in rice. For instance, rice (Oryza sativa) mutants with high levels of abscisic acid (ABA), by overexpressing rice WRKY5 (OsWRKY5), promote leaf yellowing during senescence [21]. Impairment of ABA signaling by mutation of SPOTTED LEAF3 (SPL3)-encoding rice mitogen-activated protein kinase kinase kinase 1 (OsMAPKKK1) delays leaf senescence in rice [22]. Overexpression of rice DNA-binding one zinc finger 24 (OsDOF24) represses jasmonic acid (JA) biosynthesis via the downregulation of OsAOS1, resulting in delayed leaf senescence [23]. Previous studies have reported that ethylene is associated with leaf senescence. Mutation of OsPSL, encoding a core 2/I branching beta-1,6-N-acetylglucosaminyl transferase, leads to increased endogenous ethylene level, thereby promoting leaf senescence in the psl mutant [24].
Receptor-like kinase (RLK) is one of the largest transcription factor (TF) families and comprises more than 1131 rice members [25]; it is further classified into 44 subfamilies based on the presence of N-terminal extracellular kinase domains. Of these, leucine-rich repeats (LRRs) constitute the biggest subfamily. RLKs are conserved in diverse plant species and implicated in various biological functions, such as plant development and defense; for example, maize (Zea mays) crinkly4 controls leaf development [26], Arabidopsis ERECTA regulates organ shape [27], CLAVATA 1 maintains meristems [28], HAESA is involved in floral organ abscission [29], brassinosteroid insensitive 1 (BRI1) and BRI-associated kinase 1 (BAK1) perceive brassinosteroids [30], and carrot (Daucus carota) PSKR controls cell proliferation [31]. In addition to the defined roles of RLKs in plant growth, rice (Oryza sativa) Xanthomonas resistance 21 (Xa21) enhances tolerance to Xanthomonas oryzae pv. oryzae [32], and wheat (Triticum aestivum) Lr10 locus receptor kinase (LRK10) mediates resistance to leaf rust disease [33].
In contrast to the biological functions of RLKs in plant development and defense responses, the underlying mechanisms of RLKs in leaf senescence are not clearly elucidated in rice. Herein, we report the characterization of OsSRLK, a LRR-type RLK gene, in regulating chlorophyll degradation and leaf senescence. Results showed that mutation of OsSRLK inhibited the degradation of chlorophyll and LHCII proteins during dark-induced senescence (DIS). Transcriptome analysis using RNA sequencing revealed that expression of numerous genes related to phytohormone biosynthesis and signaling was significantly altered in ossrlk mutant compared to wild type (WT) under DIS conditions. Thus, OsSRLK presumably regulates leaf senescence via regulatory pathways of chlorophyll degradation and phytohormones.

2. Results

2.1. OsSRLK Is Upregulated during Leaf Senescence

We initially investigated the spatial expression patterns of OsSRLK in the root, leaf blade, leaf sheath, stem, and panicle of the Korean japonica rice cultivar “Donjin” (hereafter termed WT), grown in the paddy field under natural long day (NLD) conditions. OsSRLK transcripts showed significantly higher accumulation in root, leaf blade, leaf sheath, and panicle than in the stem (Figure 1a). Moreover, to monitor the change of OsSRLK expression during leaf senescence, we measured the transcript levels of OsSRLK in attached and detached WT leaves, whose senescence was induced in an age-dependent manner and dark treatment, respectively. The RT-qPCR analysis revealed that OsSRLK was significantly expressed in the senescing rice leaves (Figure 1b,c). These results suggest that OsSRLK is involved in the onset and progression of leaf senescence in rice. OsSRLK comprises 3668 nucleotides, with a 1926 bp open-reading frame encoding a protein made of 641 amino acids. Amino acid sequence alignments between OsSRLK and its putative orthologs indicated that the leucine-rich repeat and catalytic domain of serine/threonine kinases were highly conserved among diverse plant species (Figure S1).

2.2. ossrlk Mutant Delays Leaf Yellowing during DIS

To examine the biological functions of OsSRLK in leaf senescence, we obtained a T-DNA insertion line (PFG_1A-15835) from the RiceGE database (http://signal.salk.edu/cgi-bin/RiceGE), in which T-DNA fragment was integrated into the 5′ untranslated region (5′ UTR) of OsSRLK (Figure 2a). To verify the expression levels of OsSRLK in this mutant line, we identified OsSRLK transcripts in the leaves of three-week-old WT and PFG_1A-15835. RT-PCR analysis revealed that, while OsSRLK transcripts highly accumulated in WT, they were lost in PFG_1A-15835 due to T-DNA insertion (Figure 2b). These results indicate that PFG_1A-15835 is a knockout mutant (hereafter termed ossrlk).
To determine the phenotypic difference between WT and ossrlk during dark-induced senescence, we observed the progress of leaf yellowing in the detached leaves of three-week-old WT and ossrlk that were incubated in 3 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (pH 5.8) at 28 °C under complete darkness. The phenotype of stay-green (sgr), a nonfunctional stay-green mutant, was used for comparison as a positive control. While WT leaves turned yellow at 5 DDI, both ossrlk and sgr leaves retained their green color (Figure 2c). In accordance with this phenotype, both Chl a and Chl b were highly accumulated in ossrlk compared to WT at 5 DDI (Figure 2d). The ratio of Chl a to Chl b was significantly less in ossrlk compared to that in WT at 5 DDI (Figure 2e). When plant cells are subjected to senescence, their reduced membrane integrity leads to an increase in the ion leakage rate [34]. Thus, ion leakage rate serves as an indicator to evaluate the plant senescence process. As presented in Figure 2f, although the ion leakage rate rapidly increased in WT after 5 DDI, only a slight change was observed in ossrlk (Figure 2f). Moreover, we investigated the levels of photosynthetic proteins via immunoblot analysis using antibodies against PSII proteins (antenna: Lhcb1 and Lhcb2; core: D1 and PsbC), PSI proteins (antenna: Lhca1, Lhca2; core: PsaA), and PAO. While PSI proteins were degraded in both WT and ossrlk at 4 DDI, the degradation of PSII proteins and PAO were inhibited in ossrlk (Figure 2g). Furthermore, we compared the chloroplast structures of ossrlk leaf tissues with those of WT at 0 and 5 DDI. Transmission electron microscopy analysis revealed that, at 0 DDI, the chloroplast structure of ossrlk was similar to that of WT; however, the thylakoids of ossrlk had bigger and thicker grana structures than those of WT at 5 DDI (Figure 2h). These results indicate that null mutation of OsSRLK delays leaf senescence by inhibiting the degradation of chlorophyll and PSII-associated LHCII during DIS.
To examine whether ossrlk delays leaf yellowing during natural senescence, we observed the senescence phenotypes in WT and ossrlk plants grown in the paddy field under natural long day conditions. While no phenotypic differences were observed between WT and ossrlk plants at one week after heading (WAH) (Figure S2a), ossrlk exhibited delayed leaf senescence phenotype at 8 WAH compared to WT (Figure S2b). We further monitored the leaf greenness and Fv/Fm ratio (efficiency of photosystem II) in WT and ossrlk plants grown in the paddy field under natural long day conditions. Based on changes in the SPAD-502 value, which determines leaf greenness, flag leaves of ossrlk retained higher green color than those of WT throughout the grain filling period (Figure S2c); however, no significant difference was observed in the change in Fv/Fm between WT and ossrlk during natural senescence under field conditions (Figure S2d). These results suggest that ossrlk delays leaf yellowing without enhanced photosynthetic capacity during natural senescence.

2.3. Overexpression of OsSRLK Accelerates Leaf Yellowing under DIS Conditions

To confirm the effects of OsSRLK on leaf yellowing, we generated three individual rice transgenic lines harboring 35S::OsSRLK. RT-qPCR analysis revealed that numerous OsSRLK transcripts were expressed in the OsSRLK-overexpressed 5 (OsSRLK-OX5) transgenic line (Figure 3a). Detached leaves of three-week-old OsSRLK-OX5 promoted leaf yellowing at 3 and 4 DDI compared to those of WT (Figure 3b). The HPLC analysis for extracts derived from detached leaves of WT, ossrlk, and OsSRLK-OX5 plants revealed that, while Chl a and Chl b was less accumulated in OsSRLK-OX5 than in WT at 0 and 4 DDI, ossrlk maintained higher green pigments during DIS compared with those in WT (Figure 3c,d). These chlorophyll contents resulted in relatively lower Chl a/b ratio in ossrlk than in WT at 4 DDI (Figure 3e). These results indicate that OsSRLK promotes leaf yellowing by degrading both Chl a and Chl b during DIS. To identify whether OsSRLK affects the expression of genes related to chlorophyll degradation and leaf senescence, we investigated the transcript levels of chlorophyll degradation genes (CDGs) and senescence-associated genes (SAGs) in the detached leaves of WT, ossrlk, and OsSRLK-OX5 plants during DIS. RT-PCR analysis revealed that no difference was observed in the transcription of CDGs and SAGs among WT, ossrlk, and OsSRLK-OX5 (Figure S3).

2.4. OsSRLK Regulates the Expression of Phytohormone-Related Genes during DIS

To understand the molecular mechanisms of OsSRLK-regulated leaf senescence, we profiled genome-wide gene expression in the detached leaves of ossrlk and WT plants using RNA sequencing (RNA-seq). Based on the criteria of (log2)-fold-change value >1 and p-value <0.05, a total of 2040 differential expressed genes (DEGs), including 737 upregulated genes and 1303 downregulated genes, were selected in ossrlk compared to those in WT during DIS. To investigate the function of DEGs, we performed gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The GO terms in 1303 downregulated DEGs were related to phytohormone signaling pathways, such as “ethylene-mediated signaling pathway”, “response to hormone stimulus”, and “auxin-mediated signaling pathway” (Figure 4a). The KEGG analysis revealed that downregulated DEGs were mainly enriched during the metabolism of glucose, amino acid, carbon, fructose, and mannose (Figure 4b). Furthermore, the MapMan analysis was used to effectively visualize the high-throughput transcriptome data [35]. The results of DEG regulation revealed genes associated with transcription, protein modification and degradation, and phytohormone biosynthesis and signaling (Figure 4c).
Thus, we carried out a literature search on phytohormonal regulation, senescence, and chlorophyll biogenesis to determine the plausible mechanisms of OsSRLK action in leaf senescence (Table 1 and Table 2).
For instance, Oryza sativa bZIP protein 8 (OSBZ8) is induced by ABA treatment and OSBZ8 binds to G-box and G-box-like sequence containing ABA-responsive elements (ABREs) [46]. Stress/ABA-activated protein kinase 6 (SAPK6), a kind of SNF1-related protein kinase 2 (SnRK2), and rice protein phosphatase 2C 51 (OsPP2C51) are involved in the ABA signaling, and their genetic modifications lead to abiotic stress tolerance and seed germination [43,48]. Lipoxygenase (LOX) oxidizes the α-linoleic acid to initiate the JA biosynthesis pathway [80]. Expression of JAmyb, a rice R2R3-type MYB transcription factor, is induced by JA treatment [57]. In particular, to identify the OsSRLK effect on the phytohormone sensitivity, we evaluated the progress of leaf degreening in the detached leaves of WT, ossrlk, and OsSRLK-OX5 plants floating on 3 mM MES (pH 5.7) solution containing 5 mM 1-aminocyclopropane-1-carboxylic acid (ACC) or 100 μM methyl jasmonate (MeJA) under continuous light conditions (Figure S4). While leaf greenness was retained for a longer duration in ossrlk than in WT at 8 days after treatment (DAT), we did not detect any phenotypic difference between WT and OsSRLK-OX5.
In addition to the involvement of OsSRLK in phytohormone biosynthesis and signaling, OsSRLK regulates the expression of genes that are related to senescence and chlorophyll biogenesis (Table 2). Overexpression of ONAC106, a rice NAC transcription factor, delays leaf senescence [76]. The rice mutant harboring nonfunctional early flowering 8 (EF8) allele, encoding a putative HAP3 subunit of the CCAAT-box-binding transcription factor, has more Chl a and Chl b compared to its parental WT [79]. Collectively, OsSRLK is involved in phytohormone biosynthesis and signaling as well as chlorophyll biogenesis.

2.5. Effects of OsSRLK on the Panicle Count Per Plant and Spikelets Per Panicle

To examine whether OsSRLK affects grain production, we evaluated yield components, including panicles per plant, spikelets per panicle, fertility, 1000-grain weight, and grain yield in WT, ossrlk, and OsSRLK-OX5 grown in the paddy field under NLD conditions. ossrlk presented more panicles per plant than WT; however, significantly lesser spikelets were present in each panicle of ossrlk compared to that of WT. These interesting traits were in contrast to the overexpression of OsSRLK; OsSRLK-OX5 revealed less panicles per plant and more spikelets per panicle (Figure 5a,b,f). In contrast to the impact of OsSRLK on the panicle and spikelet count, no significant difference was observed in the fertility and 1000-grain weight among WT, ossrlk, and OsSRLK-OX2 (Figure 5c,d). Thus, regulating the expression of OsSRLK did not improve the grain yield due to contradictory effects on the panicle and spikelet count (Figure 5e).

3. Discussion

Although the rice genome encodes numerous RLKs that regulate plant development and defense mechanisms, fewer studies are available on RLKs that participate in the regulatory pathways of leaf senescence. In the present study, we found that OsSRLK, an LRR-type RLK gene, plays a pivotal role in regulating leaf senescence. Expression of OsSRLK was induced by both age- and dark-induced leaf senescence (Figure 1b,c), and ossrlk mutation by T-DNA insertions retained the leaf greenness during DIS (Figure 2c). These observations suggest the possible roles of OsSRLK in chlorophyll degradation. Indeed, more Chl a and Chl b were present in ossrlk than in WT at 3 DDI (Figure 2d). In contrast, overexpression of OsSRLK rapidly degraded these green pigments, thereby promoting leaf yellowing compared to WT (Figure 3b,c). Both Chl a and Chl b are related to the stability of LHCII [81]. Thus, the absence of Chl b allows proteases to degrade LHCII [82]. LHCII plays a pivotal role in grana formation [3]. Based on the results obtained from microscopy and immunoblot assay (Figure 2g,h), it could be concluded that, during DIS, high levels of Chl b due to ossrlk mutation enhance the stability of LHCII and subsequently contribute to intact grana stacks.
Among the senescence regulators affecting chlorophyll degradation, CDGs encode the chlorophyll-degrading enzymes that sequentially catalyze the breakdown of chlorophyll. In the present study, CDG transcript levels in ossrlk and OsSRLK-OX5 were quite similar to those observed in WT during DIS (Figure S3), indicating that OsSRLK-regulated chlorophyll degradation is independent of regulating CDG transcription. Therefore, we explored the alternative regulatory pathways of OsSRLK-regulated chlorophyll degradation. Genome-wide analysis using RNA sequencing revealed that phytohormone-related genes were significantly altered in ossrlk compared to WT during DIS (Figure 4c). Phytohormones are an important internal factor that determines the onset and progress of leaf senescence [18]. Thus, rice and Arabidopsis plants with genetic modifications in the phytohormone-related genes exhibit delayed or promoted leaf senescence phenotype. For instance, 9-cis-epoxycarotenoid dioxygenase (NCED) is a rate-limiting enzyme involved in ABA biosynthesis, and overexpression of OsNCED3 accelerates leaf yellowing in rice [83]. Impaired ABA signal transduction by mutation in ABA insensitive 5 (ABI5) inhibits chlorophyll degradation, thereby allowing the mutants to retain leaf greenness [84,85]. In addition, JA is also considered as a senescence inducer. Inaccurate perception of JA in the rice coronatine insensitive 1 (oscoi1) mutant leads to delayed leaf yellowing [86]. Deceased endogenous JA levels due to downregulation of JA biosynthetic genes, such as LOX and allene oxide synthase (AOS), delay leaf senescence in rice [23]. Ethylene is a signaling molecule that promotes leaf senescence. For instance, increased endogenous ethylene can activate ethylene insensitive 2 (EIN2) and EIN3, resulting in precocious leaf senescence [87]. ossrlk mutation significantly downregulated the expression of phytohormone-related genes during DIS. These included OSBZ8, SAPK6, and OsPP2C51 in ABA signaling; OsLOX1, OsLOX2, and JAmyb in JA biosynthesis and signaling; and ETR2 and ETR3 in ethylene signaling (Table 1). Furthermore, the ossrlk leaves retained more green pigments than WT under ACC or MeJA treatment (Figure S4). These findings suggest that OsSRLK degrades chlorophyll by participating in the phytohormonal regulation of leaf senescence. Plants have developed several RLK signal transduction pathways to regulate phytohormone signaling. For instance, brassinosteroids are perceived by LRRs of BRI1 and form BRI1–BAK1 complex. This allows autophosphorylation on the kinase domains of BRI1, leading to BR signaling [88]. In this scenario, OsSRLK may be involved in phytohormone perception as a receptor, and it activates phytohormone signaling that mediates chlorophyll degradation (Figure S5).
Interestingly, mutation of OsSRLK increased the panicle count and decreased the spikelet count (Figure 5a,b). Tillering is an important agronomic trait that specifically determines the panicle count per plant in rice [89]. Auxin affects plant architecture, thus determining grain yield components, such as tiller number and panicle morphology [90]. The suppression of rice PIN-formed 1 (OsPIN1) RNA interference increases the tiller count [71]. The downregulation of OsPIN1 by ossrlk mutation may suggest that OsSRLK is involved in OsPIN1-mediated rice tillering (Table 2).

4. Materials and Methods

4.1. Plant Materials, Growth Conditions, and Experimental Treatments

The wild-type Oryza japonica rice cultivar “Dongjin” (parental line), ossrlk and sgr mutant plants, and OsSRLK-overexpressed transgenic plants were grown in the paddy field under NLD conditions (>14 h sunlight per day) or in a growth chamber under LD conditions (14-h light/10-h dark) in Anseong, Republic of Korea (37° N latitude). The T-DNA insertion mutant ossrlk was obtained from Kyung-Hee University, Republic of Korea [91,92]. Rice plants grown in a growth chamber for 3 weeks were used for dark and phytohormone treatments. Detached leaves of rice plants were incubated in 3 mM MES buffer (pH 5.8) with the abaxial side facing upward at 28 °C in complete darkness or in 3 mM MES buffer containing 5 mM ACC or 0.1 mM MeJA under continuous light conditions (40 mmol·m−2·s−1).

4.2. Determination of Chlorophyll Content, Photosynthetic Activity, SPAD Value, and Ion Leakage Rate

Chlorophyll was extracted from the detached leaves of three-week-old plants incubated in complete darkness using 80% ice-cold acetone. Extracts were subjected to centrifugation at 10,000× g for 15 min at 10 °C, and the absorbance of the supernatants was then measured at 647 and 663 nm using a UV–VIS spectrophotometer (BioTek instruments, Winooski, VT, USA). Chlorophyll content was calculated as previously described [93,94].
Rice plants grown in a paddy field under NLD conditions were used for determination of photosynthetic activity and SPAD value. The middle portion of the flag leaves was adapted in the dark for 10 min. The Fv/Fm ratio was measured using an OS-30p+ instrument (Opti-Science, Hudson, NH, USA). The SPAD was measured in the flag leaves using a SPAD-502 instrument (Konica Minolta, Tokyo, Japan).
Ion leakage from the detached leaves was measured by subjecting them to dark treatment as described previously with minor modifications [95]. The detached leaves were immersed in 6 mL of 400 mM mannitol for 3 h at 23 °C with gentle shaking, and the initial conductivity was determined with a conductivity meter (CON6 Meter, LaMotte Co., Chestertown, MD, USA). Total conductivity was measured after the samples were incubated at 85 °C for 20 min. The rate of ion leakage was calculated based on the ratio of the percentage of initial conductivity to that of total conductivity.

4.3. RNA Isolation and RT-qPCR Analysis

Total RNA was extracted from the leaves using the Total RNA Extraction Kit (Macrogen, Seoul, Korea), according to the manufacturer’s instructions, and RNase-free DNase (iNtRON Biotechnology, Seoul, Korea) was added to eliminate genomic DNA. First-strand cDNA was synthesized from 2 μg of total RNA in a 20 μL volume using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI, USA) and oligo(dT)15 primers and was then diluted with 80 μL distilled water. qPCR was performed with gene-specific primers, and the results were normalized to obtain rice ubiquitin 5 (OsUBQ5) (Os01g22490) (Table S1) according to the 2−ΔΔCt method [96]. The 20 μL reaction mixture included 2 μL of cDNA, 1 μL of 0.5 μM primers (Table S1), and 10 μL of 2× GoTaq master mix (Promega). The qPCR amplifications were performed using a LightCycler 480 (Roche, Basel, Switzerland) with the following conditions: 94 °C for 2 min followed by 40 cycles at 94 °C for 15 s and 60 °C for 1 min.

4.4. Transmission Electron Microscopy Analysis

Transmission electron microscopy was performed according to a previously described method with some modifications [97]. Detached leaves of three-week-old plants were incubated in complete darkness for 0 or 5 days. Small leaf pieces were fixed with modified Karnovsky’s fixative (2% paraformaldehyde, 2% glutaraldehyde, and 50 mM sodium cacodylate buffer, pH 7.2), following which the leaves were washed thrice with 50 mM sodium cacodylate buffer (pH 7.2) at 4 °C for 10 min/wash. The samples were postfixed with 1% osmium tetroxide in 50 mM sodium cacodylate buffer (pH 7.2) for 2 h at 4 °C and washed twice with distilled water at room temperature. Samples were stained en bloc in 0.5% uranyl acetate at 4 °C overnight and then dehydrated in an ethanol gradient solution with propylene oxide, followed by infiltration with Spurr’s resin. Samples were polymerized at 70 °C for 24 h and sectioned with an Ultramicrotome (MT-X, Leica, Wetzlar, Germany). The sections were mounted on copper grids and first stained with 2% uranyl acetate for 7 min and then with Reynolds’ lead citrate for 7 min. Micrographs were obtained using a LIBRA 120 transmission electron microscope (Carl Zeiss, Oberkochen, Germany).

4.5. HPLC Analysis

The green pigment was extracted according to the previously described method, and quantitative analyses for Chl a and Chl b were performed using Prominence UFLC XR (Shimadzu, Kyoto, Japan) with Brownlee SPP C18 column (4.6 × 100 mm, 2.7 um, Perkinelmer, Waltham, MA, USA). The mobile phases were separated using an elution gradient, (A) acetone/methanol (1:4, v/v) and (B) ion pair reagent/methanol (1:4, v/v), at a flow rate of 0.6 mL/min. The ion pair reagent was 1 M ammonium acetate in water; the gradient was isocratic A for 4 min, A to B for 5 min, isocratic B for 4 min, and again isocratic A for 2 min. The injected sample (5 μL) was monitored at a wavelength of 650 nm.

4.6. RNA-Seq and Analysis

Total RNA for the RNA-seq analysis was extracted from leaves of three-week-old WT and ossrlk incubated in complete darkness for 3 days. RNA-Seq was performed with an Illumina HiSeqX instrument (Illumina. San Diego, CA, USA). After trimming the low-quality bases (Q < 20) and short sequence reads (length <20), the trimmed reads were mapped onto a reference rice genome, MSU7 (RGAP, http://rice.plantbiology.msu.edu/, build 7.0), using HISAT2 software (version 2.1.0) [98]. Raw read counts were estimated using featureCounts software (Liao et al. 2013) and normalized with R package, DESeq2 [99] (R version: 3.5.0, DESeq2 version: 1.22.2). The DEGs were estimated with the software package DESeq2, and the genes that exhibited p-value < 0.05 and absolute log2 (fold change) ≥1 were determined to be significantly differentially expressed.
We retrieved GO terms related to the biological process from the rice oligonucleotide array database (http://ricephylogenomics-khu.org/ROAD_old/analysis/go_enrichment.shtml, temporary page for updating) [100]. To carry out enrichment analysis, we applied threshold query number of >2, hyper p-value < 0.05, and fold enrichment value (query number/query expected number) >2, as previously reported [101,102]. The enriched GO terms were visualized using R studio software and ggplot2 R package (R studio version: 1.1.453, ggplot2 version: 3.0.0). We conducted KEGG enrichment analysis using clusterProfiler package [103] with the organism code “dosa” and a p-value cut off of <0.05. Eventually, we visualized the result as a dot plot using the plotting function in the clusterProfiler package and additionally modified the plot using the ggplot2 package. The systemic view of the DEGs was analyzed using MapMan software (v3.6.0 RC1) [104].

4.7. Plasmid Construction and Transformation into Rice

To generate the OsSRLK-overexpression constructs, full-length OsSRLK cDNA was amplified using gene-specific primers listed in Table S1. The amplified OsSRLK cDNA was digested with the restriction enzymes HindIII and HpaI and inserted into the pGA3426 cloning vector under control of the maize ubiquitin I promoter [105]. The plasmids were transferred into Agrobacterium tumefaciens (strain LBA4404) by the freeze–thaw method and were then introduced into calli derived from Dongjin seeds via A. tumefaciens-mediated transformation [91].

4.8. SDS-PAGE and Immunoblot Analysis

Total proteins were extracted from the detached leaves of three-week-old WT and ossrlk incubated in complete darkness. Leaf tissue (10 mg) was homogenized in 100 µL of SDS sample buffer (50 mM Tris, pH 6.8, 2 mM ethylenediaminetetraacetic acid (EDTA), 10% (w/v) glycerol, 2% sodium dodecyl sulfate (SDS), and 6% 2-mercaptoethanol), and 4 µL of the protein extract was separated by 12% SDS-PAGE (w/v) and then transferred to an Immobilon-P transfer membrane (Millipore, Burlington, MA, USA). Antibodies against photosynthetic proteins (Lhca1, Lhca2, Lhcb1, Lhcb2, D1, PsaA, PsbC, and PAO) (Agrisera, Vännäs, Sweden) were used for the immunoblot analysis. Horseradish peroxidase activity of secondary antibodies (Sigma, St. Louis, MO, USA) was detected using the ECL system (AbFRONTIER, Seoul, Korea), according to the manufacturer’s instructions.

5. Conclusions

We isolated a receptor-like kinase of rice, OsSRLK, which is induced by leaf senescence. OsSRLK degraded chlorophyll and PSII proteins, thereby promoting leaf yellowing. Mutation of OsSRLK led to the downregulation of the genes related to phytohormones, senescence, and chlorophyll biogenesis. Thus, we conclude that OsSRLK acts as a positive regulator of senescence.

Supplementary Materials

The following are available online at https://www.mdpi.com/1422-0067/21/1/260/s1, Figure S1: Amino acid sequence alignment of RLK protein. Figure S2: The senescence phenotype of ossrlk under natural senescence conditions in the field. Figure S3: Expression of CDGs and SAGs in ossrlk and OsSRLK-OX5 during DIS. Figure S4: Phytohormone hyposensitivity of ossrlk. Figure S5: Proposed model for the role of OsSRLK in leaf senescence. Table S1: Primers used in this study.

Author Contributions

N.-H.S., D.T.T. and W.-J.H. performed experiments and analyzed the data. J.C. and J.-K.M. assisted in performing experiments and data analysis. K.-H.J. and Y.-H.Y. developed the plant materials. K.K., K.-H.J. and S.-C.Y. wrote and edited the manuscript. S.-C.Y. designed and supervised the project. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center, No. PJ01319601), Rural Development Administration, Republic of Korea, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2016R1D1A1A09919568 and NRF-2018R1D1A1B07050568).

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

WTwild type
Chlchlorophyll
NLDnatural long day
LDlong day
RTreverse transcriptase
DISdark-induced senescence
DDIday(s) of dark incubation
CDGschlorophyll degradation genes
SAGssenescence-associated genes
DEGsdifferential expressed genes

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Figure 1. Expression patterns of rice senescence-induced receptor-like kinase (OsSRLK). (a) OsSRLK was differentially expressed in various wild-type (WT) tissues separated from root, leaf blade, leaf sheath, stem, and panicles at the heading stage. (b,c) Transcript levels of OsSRLK were determined in the flag leaves of WT grown in the paddy field under natural long day (NLD) conditions (≥14 h light/day) (b) or in the detached leaves of WT grown in the growth chamber for three weeks under long day (LD) conditions (14 h light/10 h dark) (c). The transcript levels of OsSRLK were determined by RT-qPCR analysis and normalized to those of OsUBQ5 (AK061988). Mean and standard deviations were obtained from more than three biological replicates. Different letters indicate significant differences according to one-way ANOVA and Duncan’s least significant range test (p < 0.05). These experiments were repeated twice and gave similar results. WAH, week(s) after heading; DDI, day(s) of dark incubation.
Figure 1. Expression patterns of rice senescence-induced receptor-like kinase (OsSRLK). (a) OsSRLK was differentially expressed in various wild-type (WT) tissues separated from root, leaf blade, leaf sheath, stem, and panicles at the heading stage. (b,c) Transcript levels of OsSRLK were determined in the flag leaves of WT grown in the paddy field under natural long day (NLD) conditions (≥14 h light/day) (b) or in the detached leaves of WT grown in the growth chamber for three weeks under long day (LD) conditions (14 h light/10 h dark) (c). The transcript levels of OsSRLK were determined by RT-qPCR analysis and normalized to those of OsUBQ5 (AK061988). Mean and standard deviations were obtained from more than three biological replicates. Different letters indicate significant differences according to one-way ANOVA and Duncan’s least significant range test (p < 0.05). These experiments were repeated twice and gave similar results. WAH, week(s) after heading; DDI, day(s) of dark incubation.
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Figure 2. The ossrlk mutant shows a delay in leaf yellowing during dark-induced senescence. (a) Schematic diagram depicting the positions of T-DNA insertions in OsSRLK. Black and white bars represent the exon and untranslated region, respectively. The black line represents the intron. The triangle indicates the ossrlk mutant (PFG_1A-15835). (b) Mutation of OsSRLK verified by semiquantitative RT-PCR. OsUBQ5 was used as a loading control. The Arabic numerals on the right side of images indicate the numbers of PCR cycles. (c–f) Detached leaves of WT, ossrlk, and sgr plants grown in paddy soil for three weeks were incubated in 3 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (pH 5.8) under complete darkness at 28 °C. (c) The leaf yellowing phenotypes were observed at 0 and 5 DDI. Total chlorophyll contents (d), Chl a/b ratios (e), and ion leakage rates (f) were determined in WT and ossrlk during dark incubation. Mean and standard deviation values were obtained from the three biological repeats. Asterisks indicate a significant difference between WT and ossrlk mutant (Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001). Chl, chlorophyll; FW, fresh weight; ns, not significant. These experiments were repeated thrice with similar results. (g) Immunoblotting of detached leaves from WT and ossrlk plants at 0, 3, and 4 DDI using antibodies against photosynthetic proteins (Lhca1, Lhca2, Lhcb1, Lhcb2, D1, PsaA, PsbC, and PAO). (h) Transmission electron microscopy images were obtained from detached leaves of three-week-old WT and osdof24-D at 0 and 5 DDI, as depicted in Figure 2c. These experiments were repeated twice with similar results. G, grana (stack of thylakoids); PG, plastoglobule. Scale bars = 5 μm.
Figure 2. The ossrlk mutant shows a delay in leaf yellowing during dark-induced senescence. (a) Schematic diagram depicting the positions of T-DNA insertions in OsSRLK. Black and white bars represent the exon and untranslated region, respectively. The black line represents the intron. The triangle indicates the ossrlk mutant (PFG_1A-15835). (b) Mutation of OsSRLK verified by semiquantitative RT-PCR. OsUBQ5 was used as a loading control. The Arabic numerals on the right side of images indicate the numbers of PCR cycles. (c–f) Detached leaves of WT, ossrlk, and sgr plants grown in paddy soil for three weeks were incubated in 3 mM 2-(N-morpholino)ethanesulfonic acid (MES) buffer (pH 5.8) under complete darkness at 28 °C. (c) The leaf yellowing phenotypes were observed at 0 and 5 DDI. Total chlorophyll contents (d), Chl a/b ratios (e), and ion leakage rates (f) were determined in WT and ossrlk during dark incubation. Mean and standard deviation values were obtained from the three biological repeats. Asterisks indicate a significant difference between WT and ossrlk mutant (Student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001). Chl, chlorophyll; FW, fresh weight; ns, not significant. These experiments were repeated thrice with similar results. (g) Immunoblotting of detached leaves from WT and ossrlk plants at 0, 3, and 4 DDI using antibodies against photosynthetic proteins (Lhca1, Lhca2, Lhcb1, Lhcb2, D1, PsaA, PsbC, and PAO). (h) Transmission electron microscopy images were obtained from detached leaves of three-week-old WT and osdof24-D at 0 and 5 DDI, as depicted in Figure 2c. These experiments were repeated twice with similar results. G, grana (stack of thylakoids); PG, plastoglobule. Scale bars = 5 μm.
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Figure 3. Overexpression of OsSRLK promotes chlorophyll degradation during DIS. (a) Expression of OsSRLK in the flag leaves of WT and transgenic lines grown in the paddy field under NLD conditions. The transcript levels were determined using RT-qPCR and normalized to those of OsUBQ5. Mean and standard deviations were obtained from more than three biological replicates. Different letters indicate significant differences according to one-way ANOVA and Duncan’s least significant multiple range test (p < 0.05). (b–e) Detached leaves of WT, ossrlk, OsSRLK-OX5 plants grown in the paddy soil for three weeks were incubated in 3 mM MES (pH 5.8) under complete darkness at 28 °C. (b) The leaf yellowing phenotypes were observed at 0, 3, and 4 DDI. (c) HPLC elution profiles of chlorophyll a (Chl a) and chlorophyll b (Chl b). Absorbance at 440 nm were measured in detached leaves of WT, ossrlk, and OsSRLK-OX5 at 0 and 4 DDI. The same weight of detached leaves was extracted with the same volume of 80% acetone. The same volume of extract was loaded in each injection. Measurements of peak areas using HPLC analysis were used for determining the contents (d) and ratios (e) of Chl a and Chl b. Mean and standard deviation values were obtained from three biological repeats. Asterisks on ossrlk or OsSRLK-OX5 indicate a significant difference from WT, as determined by Student’s t-test (* p < 0.05). ns, not significant; FW, fresh weight. These experiments were repeated thrice with similar results.
Figure 3. Overexpression of OsSRLK promotes chlorophyll degradation during DIS. (a) Expression of OsSRLK in the flag leaves of WT and transgenic lines grown in the paddy field under NLD conditions. The transcript levels were determined using RT-qPCR and normalized to those of OsUBQ5. Mean and standard deviations were obtained from more than three biological replicates. Different letters indicate significant differences according to one-way ANOVA and Duncan’s least significant multiple range test (p < 0.05). (b–e) Detached leaves of WT, ossrlk, OsSRLK-OX5 plants grown in the paddy soil for three weeks were incubated in 3 mM MES (pH 5.8) under complete darkness at 28 °C. (b) The leaf yellowing phenotypes were observed at 0, 3, and 4 DDI. (c) HPLC elution profiles of chlorophyll a (Chl a) and chlorophyll b (Chl b). Absorbance at 440 nm were measured in detached leaves of WT, ossrlk, and OsSRLK-OX5 at 0 and 4 DDI. The same weight of detached leaves was extracted with the same volume of 80% acetone. The same volume of extract was loaded in each injection. Measurements of peak areas using HPLC analysis were used for determining the contents (d) and ratios (e) of Chl a and Chl b. Mean and standard deviation values were obtained from three biological repeats. Asterisks on ossrlk or OsSRLK-OX5 indicate a significant difference from WT, as determined by Student’s t-test (* p < 0.05). ns, not significant; FW, fresh weight. These experiments were repeated thrice with similar results.
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Figure 4. Transcriptome profile of the ossrlk mutant. (a) Gene ontology (GO) enrichment analysis of the 737 upregulated (Up) and 1303 downregulated (Down) differential expressed genes (DEGs) in ossrlk leaves compared to WT during dark-induced senescence (DIS). GO enrichment analysis results were visualized using the ggplot2 package. Gradual change from blue to red dots indicate gradually increased fold enrichment values. Dot size represents statistical significance (−log10(hyper p-value)). (b) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs. Dot size and color represent the ratio of selected genes to total genes in the pathway and the adjusted p-value, respectively. The numbers above the cluster indicate the DEG count for the selected KEGG pathways. (c) MapMan analysis of the DEGs. Red and blue squares indicate the members of upregulated and downregulated DEGs, respectively.
Figure 4. Transcriptome profile of the ossrlk mutant. (a) Gene ontology (GO) enrichment analysis of the 737 upregulated (Up) and 1303 downregulated (Down) differential expressed genes (DEGs) in ossrlk leaves compared to WT during dark-induced senescence (DIS). GO enrichment analysis results were visualized using the ggplot2 package. Gradual change from blue to red dots indicate gradually increased fold enrichment values. Dot size represents statistical significance (−log10(hyper p-value)). (b) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs. Dot size and color represent the ratio of selected genes to total genes in the pathway and the adjusted p-value, respectively. The numbers above the cluster indicate the DEG count for the selected KEGG pathways. (c) MapMan analysis of the DEGs. Red and blue squares indicate the members of upregulated and downregulated DEGs, respectively.
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Figure 5. OsSRLK affects the panicle count per plant and spikelets per panicle. Agronomic traits of WT, ossrlk, and OsSRLK-OX5 plants were investigated after harvest in autumn. Comparison of panicles per plant (a), spikelets per panicle (b), fertility (c), 1000-grain weight (d), yield per plant (e), and phenotype of panicles (f) between WT, ossrlk, and OsSRLK-OX5. Mean and standard deviations were obtained from 10 measurements. Asterisks on ossrlk and OsSRLK-OX5 indicate a statistically significant difference from WT, as determined by Student’s t-test (* p < 0.05, *** p < 0.001). ns, not significant.
Figure 5. OsSRLK affects the panicle count per plant and spikelets per panicle. Agronomic traits of WT, ossrlk, and OsSRLK-OX5 plants were investigated after harvest in autumn. Comparison of panicles per plant (a), spikelets per panicle (b), fertility (c), 1000-grain weight (d), yield per plant (e), and phenotype of panicles (f) between WT, ossrlk, and OsSRLK-OX5. Mean and standard deviations were obtained from 10 measurements. Asterisks on ossrlk and OsSRLK-OX5 indicate a statistically significant difference from WT, as determined by Student’s t-test (* p < 0.05, *** p < 0.001). ns, not significant.
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Table
Table 1. Hormone-related genes differentially expressed in ossrlk mutant by dark treatment.
Table 1. Hormone-related genes differentially expressed in ossrlk mutant by dark treatment.
Locus_ID (LOC_)Gene symbolFold Change (Log2)p-ValueReferences
ABA
Os02g44990MAIF12.3742860.00005[36]
Os03g57900OsiSAP71.4275660.02315[37]
Os06g33710OsSPDS21.3944060.01064[38]
Os11g08210OsNAC51.3541650.03263[39]
Os02g54160OsEREBP1−1.0196180.00204[40]
Os11g29870OsWRKY72−1.0405320.02098[41]
Os05g49890OsRAN2−1.0614540.00769[42]
Os02g34600SAPK6−1.2217920.00969[43]
Os05g02020OsRLCK176−1.3919960.00094[44]
Os05g41090OsCCaMK−1.4361170.00770[45]
Os01g46970OSBZ8−1.5106410.04456[46]
Os06g51070ONAC095−1.6219160.03699[47]
Os05g49730OsPP2C51−2.2534310.02230[48]
Os03g03370DSM2−2.4396990.00472[49]
Os07g43530OsbHLH1−2.4688230.00065[50]
Os01g01660OsIRL−4.0278660.00066[51]
JA
Os04g41680OsChia4a4.9446960.00223 [52]
Os08g38990OsWRKY301.8609350.02102 [53]
Os03g03660OsCDPK1−1.0274420.02197 [54]
Os07g30970OsNDPK1−1.1489370.00489 [55]
Os02g02840OsRacB−1.2014400.01898 [56]
Os11g45740JAmyb−1.4092070.00710 [57]
Os03g08220OsLOX2−1.5755940.00335 [58]
Os03g49380OsLOX1−2.4262670.01822 [59]
Ethylene
Os01g12900OsRac12.9521070.01944 [60]
Os01g51430OsRTH1−1.0876560.00187 [61]
Os02g57530ETR3−1.1818570.03265 [62]
Os04g08740ETR2−1.2201280.04523 [63]
Os02g57720RWC3−2.3220090.00321 [64]
Os10g28350OsARD1−3.6509230.00420 [65]
Os07g47620OsUsp1−3.8421880.00163 [66]
Auxin
Os04g38570OsABCB142.8020530.03772 [67]
Os01g53880IAA61.4869240.01248 [68]
Os04g57610OsARF12−1.2742960.03383 [69]
Os01g07500FIB−1.3956350.01820 [70]
Os02g50960OsPIN1−1.5385010.00160 [71]
Os06g48950OsARF19−1.7202150.00083 [72]
Os01g42380Ospdr9−2.0468850.00035 [73]
Os07g40290OsGH3.8−2.2658280.00652 [74]
Os05g47840OsIPT7−2.4246190.04842 [75]
Table
Table 2. Senescence-related and chlorophyll biogenesis genes differentially expressed in ossrlk mutant by dark treatment.
Table 2. Senescence-related and chlorophyll biogenesis genes differentially expressed in ossrlk mutant by dark treatment.
Locus_ID (LOC_)Gene SymbolFold Change (Log2)p-ValueReferences
Senescence
Os08g33670ONAC1060.6117180.01580[76]
Os11g29870OsWRKY72−1.0405320.02098 [41]
Os05g02020OsRLCK176−1.3919960.00094 [44]
Chlorophyll biogenesis
Os04g13540OsHFP4.0256620.00903[77]
Os06g14620RNRS1−1.2824210.03238[78]
Os08g07740EF8−2.6473140.00042[79]

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Shin, N.-H.; Trang, D.T.; Hong, W.-J.; Kang, K.; Chuluuntsetseg, J.; Moon, J.-K.; Yoo, Y.-H.; Jung, K.-H.; Yoo, S.-C. Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation. Int. J. Mol. Sci. 2020, 21, 260. https://doi.org/10.3390/ijms21010260

AMA Style

Shin N-H, Trang DT, Hong W-J, Kang K, Chuluuntsetseg J, Moon J-K, Yoo Y-H, Jung K-H, Yoo S-C. Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation. International Journal of Molecular Sciences. 2020; 21(1):260. https://doi.org/10.3390/ijms21010260

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Shin, Na-Hyun, Do Thi Trang, Woo-Jong Hong, Kiyoon Kang, Jadamba Chuluuntsetseg, Joon-Kwan Moon, Yo-Han Yoo, Ki-Hong Jung, and Soo-Cheul Yoo. 2020. "Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation" International Journal of Molecular Sciences 21, no. 1: 260. https://doi.org/10.3390/ijms21010260

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