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Open AccessArticle

Function of miR825 and miR825* as Negative Regulators in Bacillus cereus AR156-elicited Systemic Resistance to Botrytis cinerea in Arabidopsis thaliana

1
College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China
2
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
3
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5032; https://doi.org/10.3390/ijms20205032
Received: 6 August 2019 / Revised: 4 October 2019 / Accepted: 9 October 2019 / Published: 11 October 2019
(This article belongs to the Collection Feature Papers in Molecular Plant Sciences)
Small RNAs function to regulate plant defense responses to pathogens. We previously showed that miR825 and miR825* downregulate Bacillus cereus AR156 (AR156)-triggered systemic resistance to Pseudomonassyringae pv. tomato DC3000 in Arabidopsis thaliana (Arabidopsis). Here, Northern blotting revealed that miR825 and miR825* were more strongly downregulated in wild type Arabidopsis Col-0 (Col-0) plants pretreated with AR156 than in nontreated plants upon Botrytis cinerea (B. cinerea) B1301 infection. Furthermore, compared with Col-0, transgenic plants with attenuated miR825 and miR825* expression were more resistant to B. cinerea B1301, yet miR825- and miR825*-overexpressing (OE) plants were more susceptible to the pathogen. With AR156 pretreatment, the transcription of four defense-related genes (PR1, PR2, PR5, and PDF1.2) and cellular defense responses (hydrogen peroxide production and callose deposition) were faster and stronger in miR825 and miR825* knockdown lines but weaker in their OE plants than in Col-0 plants upon pathogen attack. Also, AR156 pretreatment caused stronger phosphorylation of MPK3 and MPK6 and expression of FRK1 and WRKY53 genes upon B. cinerea B1301 inoculation in miR825 and miR825* knockdown plants than in Col-0 plants. Additionally, the assay of agrobacterium-mediated transient co-expression in Nicotiana benthamiana confirmed that AT5G40910, AT5G38850, AT3G04220, and AT5G44940 are target genes of miR825 or miR825*. Compared with Col-0, the target mutant lines showed higher susceptibility to B. cinerea B1301, while still expressing AR156-triggered induced systemic resistance (ISR). The two-way analysis of variance (ANOVA) revealed a significant (P < 0.01) interactive effect of treatment and genotype on the defense responses. Hence, miR825 and miR825*act as negative regulators of AR156-mediated systemic resistance to B. cinerea B1301 in Arabidopsis. View Full-Text
Keywords: miR825 and miR825*; Bacillus cereus AR156; induced systemic resistance; Botrytis cinerea B1301; plant innate immunity miR825 and miR825*; Bacillus cereus AR156; induced systemic resistance; Botrytis cinerea B1301; plant innate immunity
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Nie, P.; Chen, C.; Yin, Q.; Jiang, C.; Guo, J.; Zhao, H.; Niu, D. Function of miR825 and miR825* as Negative Regulators in Bacillus cereus AR156-elicited Systemic Resistance to Botrytis cinerea in Arabidopsis thaliana. Int. J. Mol. Sci. 2019, 20, 5032.

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    Doi: 10.3390/ijms19071950
    Link: http://zendo.org/record/12358
    Description: Supplementary Materials: Figure S1 Plant resistance to P. capsici and CMV increased in STTM825/825* lines, but decreased in miR825/825* OE lines in comparison with that in Col-0. Figure S2 Analysis of miR825 and miR825* target mutants. Figure S3 Analysis of at5g40910 and at5g44940 mutants. Table S1 List of probes and primers used in the current research.
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