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Open AccessArticle

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

1
International Research Centre in Critical Raw Materials-ICCRAM, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain
2
Department of Chemistry, University of Burgos, Plaza Misael Bañuelos s/n., 09001 Burgos, Spain
3
Department of Molecular Cell Biology, Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(1), 205; https://doi.org/10.3390/ijms21010205
Received: 29 October 2019 / Revised: 18 December 2019 / Accepted: 24 December 2019 / Published: 27 December 2019
(This article belongs to the Special Issue Graphene-Based Materials: Biological and Biomedical Applications)
The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial. View Full-Text
Keywords: graphene; unicellular organisms; toxicity; binding capacity; ATR-FTIR; TEM; ICP-MS graphene; unicellular organisms; toxicity; binding capacity; ATR-FTIR; TEM; ICP-MS
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MDPI and ACS Style

Domi, B.; Rumbo, C.; García-Tojal, J.; Elena Sima, L.; Negroiu, G.; Tamayo-Ramos, J.A. Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules. Int. J. Mol. Sci. 2020, 21, 205. https://doi.org/10.3390/ijms21010205

AMA Style

Domi B, Rumbo C, García-Tojal J, Elena Sima L, Negroiu G, Tamayo-Ramos JA. Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules. International Journal of Molecular Sciences. 2020; 21(1):205. https://doi.org/10.3390/ijms21010205

Chicago/Turabian Style

Domi, Brixhilda; Rumbo, Carlos; García-Tojal, Javier; Elena Sima, Livia; Negroiu, Gabriela; Tamayo-Ramos, Juan A. 2020. "Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules" Int. J. Mol. Sci. 21, no. 1: 205. https://doi.org/10.3390/ijms21010205

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