Next Article in Journal
The Impact of Caloric Restriction on the Epigenetic Signatures of Aging
Previous Article in Journal
Improvement of l-Leucine Production in Corynebacterium glutamicum by Altering the Redox Flux
Open AccessArticle

Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant

1
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
2
Institute of Crop Genetics and Breeding, Yangtze University, Jingzhou 434025, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(8), 2021; https://doi.org/10.3390/ijms20082021
Received: 20 March 2019 / Revised: 20 April 2019 / Accepted: 22 April 2019 / Published: 24 April 2019
(This article belongs to the Section Molecular Plant Sciences)
Fagopyrum esculentum (Polygonaceae: Caryophyllales) exhibits an undifferentiated perianth comprising five showy tepals, which does not completely correspond to the perianth differentiated into typical sepals and petals in most core eudicots. In Arabidopsis, the APETALA1 (AP1) gene is involved in specifying sepals and petals development. Here we isolated AP1 ortholog, FaesAP1, and a 2.2kb FaesAP1 promoter (pFaesAP1) from F. esculentum. FaesAP1 expression is mainly detectable in all floral organs and maintains at a high level when tepals elongate rapidly both in pin and thrum flowers. Moreover, the GUS reporter gene driven by pFaesAP1 was activated in flowers where the sepals were intense, but the petals very weak or absent. Additionally, FaesAP1 ectopic expression in Arabidopsis ap1-10 mutant rescues sepal development fully, obviously prompting early flowering, but failing to complement petal development. In this study, evidence was provided that the showy tepals in the F. esculentum are homologs to core eudicots sepals. Furthermore, these findings show a different perianth identity program in Caryophyllales, suggesting that AP1 orthologs involved in petal development may evolve independently across different clades of core eudicots. Our results also suggest that FaesAP1 holds potential for biotechnical engineering to develop early flowering varieties of F. esculentum. View Full-Text
Keywords: APETALA1; Fagopyrum esculentum; floral development; flowering; perianth development APETALA1; Fagopyrum esculentum; floral development; flowering; perianth development
Show Figures

Figure 1

MDPI and ACS Style

Liu, Z.; Fei, Y.; Zhang, K.; Fang, Z. Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant. Int. J. Mol. Sci. 2019, 20, 2021.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop