Individual Immune-Modulatory Capabilities of MSC-Derived Extracellular Vesicle (EV) Preparations and Recipient-Dependent Responsiveness
Abstract
:1. Introduction
2. Results
2.1. Induction of T cell Differentiation and Cytokine Production Upon PMA/Ionomycin Stimulation
2.2. Modulation of CD45RA Expression on T cells by MSC-EVs upon PMA/Ionomycin Stimulation
2.3. Modulation of the Cytokine Response of T cells Subsets by MSC-EVs upon PMA/Ionomycin Stimulation
2.4. Influence of PBMC Donor Heterogeneity during MSC-EV Administration Upon PMA/Ionomycin Stimulation
3. Discussion
4. Materials and Methods
4.1. Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stem Cells
4.2. Stimulation of PBMC
4.3. Flow Cytometric Analysis
4.4. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kordelas, L.; Schwich, E.; Dittrich, R.; Horn, P.A.; Beelen, D.W.; Börger, V.; Giebel, B.; Rebmann, V. Individual Immune-Modulatory Capabilities of MSC-Derived Extracellular Vesicle (EV) Preparations and Recipient-Dependent Responsiveness. Int. J. Mol. Sci. 2019, 20, 1642. https://doi.org/10.3390/ijms20071642
Kordelas L, Schwich E, Dittrich R, Horn PA, Beelen DW, Börger V, Giebel B, Rebmann V. Individual Immune-Modulatory Capabilities of MSC-Derived Extracellular Vesicle (EV) Preparations and Recipient-Dependent Responsiveness. International Journal of Molecular Sciences. 2019; 20(7):1642. https://doi.org/10.3390/ijms20071642
Chicago/Turabian StyleKordelas, Lambros, Esther Schwich, Robin Dittrich, Peter A. Horn, Dietrich W. Beelen, Verena Börger, Bernd Giebel, and Vera Rebmann. 2019. "Individual Immune-Modulatory Capabilities of MSC-Derived Extracellular Vesicle (EV) Preparations and Recipient-Dependent Responsiveness" International Journal of Molecular Sciences 20, no. 7: 1642. https://doi.org/10.3390/ijms20071642