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Open AccessArticle

Analysis of Rice Proteins with DLN Repressor Motif/S

1
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi-110067, India
2
Department of Plant Molecular Biology, South Campus Delhi University, New Delhi-110021, India
*
Author to whom correspondence should be addressed.
These authors contributed equally to the manuscript.
Int. J. Mol. Sci. 2019, 20(7), 1600; https://doi.org/10.3390/ijms20071600
Received: 16 January 2019 / Revised: 25 January 2019 / Accepted: 31 January 2019 / Published: 30 March 2019
(This article belongs to the Special Issue Transcriptional and Post-transcriptional Gene Regulation in Plants)
Transcriptional regulation includes both activation and repression of downstream genes. In plants, a well-established class of repressors are proteins with an ERF-associated amphiphilic repression/EAR domain. They contain either DLNxxP or LxLxL as the identifying hexapeptide motif. In rice (Oryza sativa), we have identified a total of 266 DLN repressor proteins, with the former motif and its modifications thereof comprising 227 transcription factors and 39 transcriptional regulators. Apart from DLNxxP motif conservation, DLNxP and DLNxxxP motifs with variable numbers/positions of proline and those without any proline conservation have been identified. Most of the DLN repressome proteins have a single DLN motif, with higher relative percentage in the C-terminal region. We have designed a simple yeast-based experiment wherein a DLN motif can successfully cause strong repression of downstream reporter genes, when fused to a transcriptional activator of rice or yeast. The DLN hexapeptide motif is essential for repression, and at least two “DLN” residues cause maximal repression. Comparatively, rice has more DLN repressor encoding genes than Arabidopsis, and DLNSPP motif from rice is 40% stronger than the known Arabidopsis SRDX motif. The study reports a straightforward assay to analyze repressor activity, along with the identification of a strong DLN repressor from rice. View Full-Text
Keywords: EAR motif; rice; seed development; trans-repression; yeast EAR motif; rice; seed development; trans-repression; yeast
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MDPI and ACS Style

Singh, P.; Mathew, I.E.; Verma, A.; Tyagi, A.K.; Agarwal, P. Analysis of Rice Proteins with DLN Repressor Motif/S. Int. J. Mol. Sci. 2019, 20, 1600. https://doi.org/10.3390/ijms20071600

AMA Style

Singh P, Mathew IE, Verma A, Tyagi AK, Agarwal P. Analysis of Rice Proteins with DLN Repressor Motif/S. International Journal of Molecular Sciences. 2019; 20(7):1600. https://doi.org/10.3390/ijms20071600

Chicago/Turabian Style

Singh, Purnima; Mathew, Iny E.; Verma, Ankit; Tyagi, Akhilesh K.; Agarwal, Pinky. 2019. "Analysis of Rice Proteins with DLN Repressor Motif/S" Int. J. Mol. Sci. 20, no. 7: 1600. https://doi.org/10.3390/ijms20071600

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