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Article

Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA

by
Alexandra S. Dubrovina
1,*,
Olga A. Aleynova
1,
Alexander V. Kalachev
2,
Andrey R. Suprun
1,3,
Zlata V. Ogneva
1 and
Konstantin V. Kiselev
1,3
1
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690022, Russia
2
Laboratory of Embryology, National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690041, Russia
3
Far Eastern Federal University, The School of Natural Sciences, Vladivostok 690090, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(7), 1585; https://doi.org/10.3390/ijms20071585
Submission received: 1 February 2019 / Revised: 19 March 2019 / Accepted: 26 March 2019 / Published: 29 March 2019
(This article belongs to the Special Issue Transcriptional and Post-transcriptional Gene Regulation in Plants)
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Abstract

Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome can undergo suppression as a result of exogenous RNA application on plant surface is almost unexplored. This study analyzed whether it is possible to influence transcript levels of transgenes, as more prone sequences to silencing, in Arabidopsis genome by direct exogenous application of target long dsRNAs. The data revealed that in vitro synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (EGFP) or neomycin phosphotransferase II (NPTII) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.
Keywords: dsRNA; transgene; gene silencing; external RNA application; RNA interference; Arabidopsis thaliana dsRNA; transgene; gene silencing; external RNA application; RNA interference; Arabidopsis thaliana

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MDPI and ACS Style

Dubrovina, A.S.; Aleynova, O.A.; Kalachev, A.V.; Suprun, A.R.; Ogneva, Z.V.; Kiselev, K.V. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. Int. J. Mol. Sci. 2019, 20, 1585. https://doi.org/10.3390/ijms20071585

AMA Style

Dubrovina AS, Aleynova OA, Kalachev AV, Suprun AR, Ogneva ZV, Kiselev KV. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. International Journal of Molecular Sciences. 2019; 20(7):1585. https://doi.org/10.3390/ijms20071585

Chicago/Turabian Style

Dubrovina, Alexandra S., Olga A. Aleynova, Alexander V. Kalachev, Andrey R. Suprun, Zlata V. Ogneva, and Konstantin V. Kiselev. 2019. "Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA" International Journal of Molecular Sciences 20, no. 7: 1585. https://doi.org/10.3390/ijms20071585

APA Style

Dubrovina, A. S., Aleynova, O. A., Kalachev, A. V., Suprun, A. R., Ogneva, Z. V., & Kiselev, K. V. (2019). Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. International Journal of Molecular Sciences, 20(7), 1585. https://doi.org/10.3390/ijms20071585

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