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Open AccessArticle

Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis

1
Chair and Department of Food and Nutrition, Medical University of Lublin, 4a Chodźki Str., 20-093 Lublin, Poland
2
Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, 1 Chodźki, Lublin 20-093, Poland
3
Department of Cosmetology, University of Information Technology and Management in Rzeszow, 35-225 Rzeszów, Poland
4
Chair and Department of Pharmacognosy with Medicinal Plants Unit, Medical University of Lublin, 20-093 Lublin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(4), 898; https://doi.org/10.3390/ijms20040898
Received: 16 January 2019 / Revised: 2 February 2019 / Accepted: 11 February 2019 / Published: 19 February 2019
(This article belongs to the Special Issue Curcumin in Health and Disease)
Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) based bioautography studies to reveal its antimicrobial constituents and construct a universal platform for the bioactivity assessment of crude extracts, with help of a freeware ImageJ software. This optimized chromatographic bioassay performed on diethyl ether and methanol extracts of Curcuma longa was successfully applied on the total extract and revealed the antimicrobial potential of single components against a variety of Gram-positive strains, with no need for their isolation from the mixture. The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) (v/v/v/v). View Full-Text
Keywords: Curcuma longa; turmeric tuber; Zingiberaceae; TLC bioautography; antimicrobial agents; ImageJ; TLC-MS; hydrostatic counter-current chromatography; centrifugal partition chromatography Curcuma longa; turmeric tuber; Zingiberaceae; TLC bioautography; antimicrobial agents; ImageJ; TLC-MS; hydrostatic counter-current chromatography; centrifugal partition chromatography
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MDPI and ACS Style

Czernicka, L.; Grzegorczyk, A.; Marzec, Z.; Antosiewicz, B.; Malm, A.; Kukula-Koch, W. Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis. Int. J. Mol. Sci. 2019, 20, 898.

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