Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model
by
Nesrine Ebrahim 1,2, Yasser Mohammad Hassan Mandour 3, Ayman Samir Farid 4,*
, Ebtesam Nafie 5, Amira Zaky Mohamed 6, Miriam Safwat 7, Radwa Taha 7, Dina Sabry 8,9, Safwa M. Sorour 10 and Ahmed Refae 3
Nesrine Ebrahim 1,2, Yasser Mohammad Hassan Mandour 3, Ayman Samir Farid 4,*, Ebtesam Nafie 5, Amira Zaky Mohamed 6, Miriam Safwat 7, Radwa Taha 7, Dina Sabry 8,9, Safwa M. Sorour 10 and Ahmed Refae 3
1
Department of Histology and Cell Biology, Benha University, Benha, Qalyubia 13518, Egypt
2
Stem Cell Unit, Benha University, Benha, Qalyubia 13518, Egypt
3
Department of Otorhinolaryngology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt
4
Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalyubia 13736, Egypt
5
Zoology Department, Faculty of Science, Benha University, Benha 13518, Egypt
6
Department of Microbiology, Faculty of Science, Tanta University, Tanta 31527, Egypt
7
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
8
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
9
Molecular Biology and Stem Cell Unit, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
10
Department of Clinical Pharmacology, Faculty of Medicine, Benha University, Benha, Qalyubia 13518, Egypt
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(4), 873; https://doi.org/10.3390/ijms20040873
Received: 30 January 2019 / Revised: 13 February 2019 / Accepted: 14 February 2019 / Published: 18 February 2019
(This article belongs to the Special Issue Stem Cell-Based Therapy)
This study was designed to investigate the potential effects and underlying mechanism of adipose tissue-derived mesenchymal stem cells (MSCs) on allergic inflammation compared to Montelukast as an antileukotriene drug in a rat model of allergic rhinitis (AR). The effect of MSCs was evaluated in albino rats that were randomly divided into four (control, AR, AR + Montelukast, and AR + MSCs) groups. Rats of AR group were sensitized by ovalbumin (OVA) and then challenged with daily nasal drops of OVA diluted in sterile physiological saline (50 μL/nostril, 100 mg/mL, 10% OVA) from day 15 to day 21 of treatment with/without Montelukast (1 h before each challenge) or MSCs I/P injection (1 × 106 MCSs; weekly for three constitutive weeks). Both Montelukast and MSCs treatment started from day 15 of the experiment. At the end of the 5th week, blood samples were collected from all rats for immunological assays, histological, and molecular biology examinations. Both oral Montelukast and intraperitoneal injection of MSCs significantly reduced allergic symptoms and OVA-specific immunoglobulin E (IgE), IgG1, IgG2a and histamine as well as increasing prostaglandin E2 (PGE2). Further analysis revealed that induction of nasal innate cytokines, such as interleukin (IL)-4 and TNF-α; and chemokines, such as CCL11 and vascular cell adhesion molecule-1 (VCAM-1), were suppressed; and transforming growth factor-β (TGF-β) was up-regulated in Montelukast and MSCs-treated groups with superior effect to MSCs, which explained their underlying mechanism. In addition, the adipose tissue-derived MSCs-treated group had more restoring effects on nasal mucosa structure demonstrated by electron microscopical examination.
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Keywords:
allergic rhinitis; antileukotriene; CCL11; immunoglobulins; mesenchymal stem cells; Montelukast; VCAM-1
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MDPI and ACS Style
Ebrahim, N.; Mandour, Y.M.H.; Farid, A.S.; Nafie, E.; Mohamed, A.Z.; Safwat, M.; Taha, R.; Sabry, D.; Sorour, S.M.; Refae, A. Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model. Int. J. Mol. Sci. 2019, 20, 873.
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