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Int. J. Mol. Sci. 2019, 20(3), 768; https://doi.org/10.3390/ijms20030768

IL-10 Could Play a Role in the Interrelation between Diabetes Mellitus and Osteoarthritis

1
Institute of Anatomy, Paracelsus Medical University, Nuremberg and Salzburg, Prof. Ernst Nathan Str. 1, 90419 Nuremberg, Germany
2
Department of Biosciences, Paris Lodron University Salzburg, Hellbrunnerstr. 34, 5020 Salzburg, Austria
3
Department of Orthopedics and Trauma Surgery, Nuremberg General Hospital, Paracelsus Medical University, Nueremberg. Breslauer Strasse 201, 90471 Nuremberg, Germany
*
Author to whom correspondence should be addressed.
Single senior author.
Received: 27 December 2018 / Revised: 19 January 2019 / Accepted: 31 January 2019 / Published: 12 February 2019
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Abstract

The association between osteoarthritis (OA), obesity and metabolic syndrome suggests an interrelation between OA and diabetes mellitus (DM). Little is known about the role of anti-inflammatory cytokine interleukin (IL)-10 in the interrelation between OA and DM. Hence, the effects of IL-10 under hyperglycemia (HG) and hyperinsulinemia (HI) in human articular chondrocytes (hAC) and chondrosarcoma cell line Okayama University Medical School (OUMS)-27 were examined. HAC and OUMS-27, cultured in normoglycemic (NG) and HG conditions were stimulated with insulin and/or IL-10. Cell survival, metabolic activity, proliferation and extracellular matrix (ECM) synthesis were immunocytochemically examined. No significant differences in vitality of hAC neither in pure NG (NGw/o) nor HG (HGw/o) conditions were found. Applying HI and/or IL-10 in both conditions reduced significantly the vitality of hAC but not of OUMS-27. HG impaired significantly hAC metabolism. When combined with HI + IL-10 or IL-10 alone it decreased also significantly hAC proliferation compared to NGw/o. In OUMS-27 it induced only a trend of impaired proliferation compared to NGw/o. hAC but not OUMS-27 reduced significantly their collagen type (col) I, SOX9 and proteoglycan (PG) synthesis in HG combined with HI +/− IL-10 compared to NGw/o. IL-10 could not moderate HI and HG effects. In contrast to hAC OUMS-27 showed limited sensitivity as DM model. View Full-Text
Keywords: osteoarthritis; diabetes mellitus; hyperglycemia; hyperinsulinemia; IL-10; human articular chondrocytes osteoarthritis; diabetes mellitus; hyperglycemia; hyperinsulinemia; IL-10; human articular chondrocytes
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Silawal, S.; Willauschus, M.; Schulze-Tanzil, G.; Gögele, C.; Geßlein, M.; Schwarz, S. IL-10 Could Play a Role in the Interrelation between Diabetes Mellitus and Osteoarthritis. Int. J. Mol. Sci. 2019, 20, 768.

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