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Int. J. Mol. Sci. 2019, 20(3), 767; https://doi.org/10.3390/ijms20030767

MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells

1
Oral and Maxillofacial Diagnostic Sciences, University of Florida College of Dentistry, Gainesville, FL 32610, USA
2
Oral Biology, University of Florida College of Dentistry, Gainesville, FL 32610, USA
3
Pathology, University of Florida College of Medicine, Gainesville, FL 32610, USA
4
Pharmacology, University of North Carolina College of Medicine, Chapel Hill, NC 27599, USA
*
Author to whom correspondence should be addressed.
Received: 23 January 2019 / Revised: 5 February 2019 / Accepted: 5 February 2019 / Published: 12 February 2019
(This article belongs to the Special Issue Role and Application of Stem Cells in Regenerative Medicine)
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Abstract

Sjögren’s syndrome (SjS) is an autoimmune disease that destroys the salivary glands and results in severe dry mouth. Mesenchymal stem cell (MSC) transplantation has been recently proposed as a promising therapy for restoring cells in multiple degenerative diseases. We have recently utilized advanced proteomics biochemical assays to identify the key molecules involved in the mesenchymal-epithelial transition (MET) of co-cultured mouse bone-marrow-derived MSCs mMSCs with primary salivary gland cells. Among the multiple transcription factors (TFs) that were differentially expressed, two major TFs were selected: muscle, intestine, and stomach expression-1 (MIST1) and transcription factor E2a (TCF3). These factors were assessed in the current study for their ability to drive the expression of acinar cell marker, alpha-salivary amylase 1 (AMY1), and ductal cell marker, cytokeratin19 (CK19), in vitro. Overexpression of MIST1-induced AMY1 expression while it had little effect on CK19 expression. In contrast, TCF3 induced neither of those cellular markers. Furthermore, we have identified that mMSCs express muscarinic-type 3 receptor (M3R) mainly in the cytoplasm and aquaporin 5 (AQP5) in the nucleus. While MIST1 did not alter M3R levels in mMSCs, a TCF3 overexpression downregulated M3R expressions in mMSCs. The mechanisms for such differential regulation of glandular markers by these TFs warrant further investigation. View Full-Text
Keywords: mouse bone-marrow-mesenchymal stem cells; alpha-salivary amylase 1; MIST1; TCF3; Sjögren’s syndrome; salivary precursors mouse bone-marrow-mesenchymal stem cells; alpha-salivary amylase 1; MIST1; TCF3; Sjögren’s syndrome; salivary precursors
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Mona, M.; Miller, R.; Li, H.; Park, Y.-J.; Zaman, R.; Yang, L.-J.; Cha, S. MIST1, an Inductive Signal for Salivary Amylase in Mesenchymal Stem Cells. Int. J. Mol. Sci. 2019, 20, 767.

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