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Open AccessArticle

Comparative Transcriptome Analysis between a Novel Allohexaploid Cotton Progeny CMS Line LD6A and Its Maintainer Line LD6B

1
Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning 530006, China
2
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6127; https://doi.org/10.3390/ijms20246127
Received: 10 October 2019 / Revised: 28 November 2019 / Accepted: 1 December 2019 / Published: 5 December 2019
(This article belongs to the Section Molecular Plant Sciences)
Cytoplasmic male sterility (CMS) is an important agronomic feature and provides an effective tool for heterosis utilization of crops. This study reports the comparative transcriptomic sketches between a novel allohexaploid cotton progeny CMS line LD6A and its maintainer line LD6B using de novo transcriptome sequencing technology at the pollen abortion stage. A total of 128,901 Unigenes were identified, in which 2007 were upregulated and 11,864 were downregulated. The significantly differentially expressed genes (DEGs) in LD6A show a distant and diverse genetic nature due to their distant hybrid hexaploidy progeny. Further analysis revealed that most of the DEGs participated in the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, histone acetyltransferase activity, sepal development, stigma development, cotyledon development and microsporogenesis. A highly differentially expressed toxic protein, Abrin, was identified in the CMS line LD6A, which can catalyze the inactivation of ribosomes and consequently lead to cell death through the mitochondrial pathway in human cells. Twelve DEGs were selected randomly to validate transcriptome data using quantitative reverse-transcribed PCR (qRT-PCR). This study will contribute to new ideas and foundations related to the molecular mechanism of CMS and the innovation of cotton germplasm resources. View Full-Text
Keywords: cotton; allohexaploid; cytoplasmic male sterility; transcriptome; differentially expressed genes cotton; allohexaploid; cytoplasmic male sterility; transcriptome; differentially expressed genes
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MDPI and ACS Style

Zheng, J.; Kong, X.; Li, B.; Khan, A.; Li, Z.; Liu, Y.; Kang, H.; Ullah Dawar, F.; Zhou, R. Comparative Transcriptome Analysis between a Novel Allohexaploid Cotton Progeny CMS Line LD6A and Its Maintainer Line LD6B. Int. J. Mol. Sci. 2019, 20, 6127.

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