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Open AccessReview

Dead Cas Systems: Types, Principles, and Applications

1
National Medical Research Center of Tuberculosis and Infectious Diseases, Ministry of Health, Moscow 127994, Russia
2
Institute of Immunology, Federal Medical Biological Agency, Moscow 115522, Russia
3
Sechenov First Moscow State Medical University, Moscow 119146, Russia
4
Central Research Institute of Epidemiology, Moscow 111123, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6041; https://doi.org/10.3390/ijms20236041
Received: 31 October 2019 / Revised: 26 November 2019 / Accepted: 28 November 2019 / Published: 30 November 2019
(This article belongs to the Section Molecular Biology)
The gene editing tool CRISPR-Cas has become the foundation for developing numerous molecular systems used in research and, increasingly, in medical practice. In particular, Cas proteins devoid of nucleolytic activity (dead Cas proteins; dCas) can be used to deliver functional cargo to programmed sites in the genome. In this review, we describe current CRISPR systems used for developing different dCas-based molecular approaches and summarize their most significant applications. We conclude with comments on the state-of-art in the CRISPR field and future directions. View Full-Text
Keywords: Cas9; dCas; transcription; epigenetics; chromatin; cancer; hereditary diseases; inflammatory diseases; infectious diseases; editing Cas9; dCas; transcription; epigenetics; chromatin; cancer; hereditary diseases; inflammatory diseases; infectious diseases; editing
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Brezgin, S.; Kostyusheva, A.; Kostyushev, D.; Chulanov, V. Dead Cas Systems: Types, Principles, and Applications. Int. J. Mol. Sci. 2019, 20, 6041.

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