miR-7 Knockdown by Peptide Nucleic Acids in the Ascidian Ciona intestinalis
Abstract
:1. Introduction
2. Results
2.1. miR-7 in Ciona intestinalis
2.2. Genes Expression Profile
2.3. miR-7 Downregulation by PNAs
3. Discussion
4. Materials and Methods
4.1. Animals and Embryos Culture
4.2. Reagents
4.3. PNAs Synthesis and Characterization
4.4. Microinjections
4.5. Whole Mount In Situ Hybridization
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
ASWH | Artificial Sea Water with HEPES |
AmiR-7 | AntagomiR anti-miR-7 |
Boc | tert-Butyloxycarbonyl |
ESI-Q-TOF MS | Electrospray-ionisation quadrupole time-of-flight mass spectrometry |
LNA | Locked Nucleic Acid |
MBHA | 4-Methylbenzhydrylamine hydrochloride |
PNA | Peptide Nucleic Acid |
PNA-a7 | Peptide Nucleic Acid anti-miR-7 |
PNA-sc7 | Peptide Nucleic Acid scrambled sequence |
RP-HPLC | Reverse phase-high pressure liquid chromatography |
Z | Benzyloxycarbonyl |
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Mercurio, S.; Cauteruccio, S.; Manenti, R.; Candiani, S.; Scarì, G.; Licandro, E.; Pennati, R. miR-7 Knockdown by Peptide Nucleic Acids in the Ascidian Ciona intestinalis. Int. J. Mol. Sci. 2019, 20, 5127. https://doi.org/10.3390/ijms20205127
Mercurio S, Cauteruccio S, Manenti R, Candiani S, Scarì G, Licandro E, Pennati R. miR-7 Knockdown by Peptide Nucleic Acids in the Ascidian Ciona intestinalis. International Journal of Molecular Sciences. 2019; 20(20):5127. https://doi.org/10.3390/ijms20205127
Chicago/Turabian StyleMercurio, Silvia, Silvia Cauteruccio, Raoul Manenti, Simona Candiani, Giorgio Scarì, Emanuela Licandro, and Roberta Pennati. 2019. "miR-7 Knockdown by Peptide Nucleic Acids in the Ascidian Ciona intestinalis" International Journal of Molecular Sciences 20, no. 20: 5127. https://doi.org/10.3390/ijms20205127