Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Identification and Phylogenetic Analysis of Tomato Swi3-Like Proteins
2.2. Subcellular Localization of Tomato Swi3-Like Proteins
2.3. The Expression Patterns of Tomato Swi3-Like Genes
2.4. Members of SlSWI3s Interact with Other Proteins
2.5. Ectopic Overexpression of SlSWI3C Enhances Leaf Growth
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Identification of Tomato Swi3-Like Genes and Phylogenetic Tree Construction
4.3. Subcellular Localization Assays
4.4. Yeast-Two-Hybrid Assay
4.5. LCI Assay
4.6. Co-Immunoprecipitation (Co-IP) Assays
4.7. RNA Extraction and Expression Analyses
4.8. Generation of Transgenic Plants
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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SWI3 Gene Family | Gene Name | Gene Code | Accession Number a | ORF Length b | Protein Length | Localization c | Number of Exons | Nuclear Localization Signal d |
---|---|---|---|---|---|---|---|---|
SWI3A | SlSWI3A | Solyc03g097450 | XP_004235292 | 1772 | 574 | nucl, cyto | 7 | Yes |
AtSWI3A | AT2G47620 | NP_850476 | 1539 | 513 | nucl, cyto | 7 | Yes | |
OsSWI3A | LOC_Os04g40420 | BAF15014 | 1683 | 561 | nucl, cyto, plas | 7 | Yes | |
SWI3B | SlSWI3B | Solyc04g082760 | XP_004238459 | 1470 | 490 | nucl, cyto | 6 | Yes |
AtSWI3B | AT2G33610 | NP_180919 | 1410 | 469 | nucl, cyto | 6 | Yes | |
OsSWI3B | LOC_Os02g10060 | XP_015625317 | 1536 | 512 | nucl | 6 | Yes | |
SWI3C | SlSWI3C | Solyc06g060120 | XP_004242041 | 2376 | 792 | nucl, chlo, mito | 8 | Yes |
AtSWI3C | AT1G21700 | NP_173589 | 2424 | 807 | nucl, chlo, plas | 9 | Yes | |
OsSWI3C1 | LOC_Os12g07730 | ABA96607 | 2520 | 840 | nucl, chlo, mito | 9 | Yes | |
OsSWI3C2 | LOC_Os11g08080 | XP_015617508 | 2355 | 785 | nucl, chlo, mito | 9 | Yes | |
SWI3D | SlSWI3D | Solyc01g109510 | XP_004230866 | 2838 | 946 | nucl | 6 | Yes |
AtSWI3D | AT4G34430 | NP_974682 | 2961 | 986 | nucl | 7 | Yes | |
OsSWI3D1 | LOC_Os03g51220 | XP_015632283 | 2745 | 915 | nucl, cyto | 8 | Yes | |
OsSWI3D2 | LOC_Os04g01970 | XP_015633469 | 2661 | 887 | nucl, chlo, mito | 7 | Yes |
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Zhao, Z.; Li, T.; Peng, X.; Wu, K.; Yang, S. Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis. Int. J. Mol. Sci. 2019, 20, 5121. https://doi.org/10.3390/ijms20205121
Zhao Z, Li T, Peng X, Wu K, Yang S. Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis. International Journal of Molecular Sciences. 2019; 20(20):5121. https://doi.org/10.3390/ijms20205121
Chicago/Turabian StyleZhao, Zhongyi, Tao Li, Xiuling Peng, Keqiang Wu, and Songguang Yang. 2019. "Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis" International Journal of Molecular Sciences 20, no. 20: 5121. https://doi.org/10.3390/ijms20205121