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Open AccessArticle

Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis

by 1,2,3,†, 4,†, 2,5, 6,* and 2,*
1
Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China
2
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
3
College of Life Sciences, China West Normal University, Nanchong 637002, China
4
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510650, China
5
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China
6
Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(20), 5121; https://doi.org/10.3390/ijms20205121
Received: 12 September 2019 / Revised: 9 October 2019 / Accepted: 10 October 2019 / Published: 16 October 2019
(This article belongs to the Special Issue Chromatin, Epigenetics and Plant Physiology)
As the subunits of the SWI/SNF (mating-type switching (SWI) and sucrose nonfermenting (SNF)) chromatin-remodeling complexes (CRCs), Swi3-like proteins are crucial to chromatin remodeling in yeast and human. Growing evidence indicate that AtSWI3s are also essential for development and response to hormones in Arabidopsis. Nevertheless, the biological functions of Swi3-like proteins in tomato (Solanum lycopersicum) have not been investigated. Here we identified four Swi3-like proteins from tomato, namely SlSWI3A, SlSWI3B, SlSWI3C, and SlSWI3D. Subcellular localization analysis revealed that all SlSWI3s are localized in the nucleus. The expression patterns showed that all SlSWI3s are ubiquitously expressed in all tissues and organs, and SlSWI3A and SlSWI3B can be induced by cold treatment. In addition, we found that SlSWI3B can form homodimers with itself and heterodimers with SlSWI3A and SlSWI3C. SlSWI3B can also interact with SlRIN and SlCHR8, two proteins involved in tomato reproductive development. Overexpression of SlSWI3C increased the leaf size in transgenic Arabidopsis with increased expression of GROWTH REGULATING FACTORs, such as GRF3, GRF5, and GRF6. Taken together, our results indicate that SlSWI3s may play important roles in tomato growth and development. View Full-Text
Keywords: Swi3-like proteins; gene expression; protein interaction; leaf development; tomato Swi3-like proteins; gene expression; protein interaction; leaf development; tomato
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MDPI and ACS Style

Zhao, Z.; Li, T.; Peng, X.; Wu, K.; Yang, S. Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis. Int. J. Mol. Sci. 2019, 20, 5121. https://doi.org/10.3390/ijms20205121

AMA Style

Zhao Z, Li T, Peng X, Wu K, Yang S. Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis. International Journal of Molecular Sciences. 2019; 20(20):5121. https://doi.org/10.3390/ijms20205121

Chicago/Turabian Style

Zhao, Zhongyi; Li, Tao; Peng, Xiuling; Wu, Keqiang; Yang, Songguang. 2019. "Identification and Characterization of Tomato SWI3-Like Proteins: Overexpression of SlSWIC Increases the Leaf Size in Transgenic Arabidopsis" Int. J. Mol. Sci. 20, no. 20: 5121. https://doi.org/10.3390/ijms20205121

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