Evolution of the Proto Sex-Chromosome in Solea senegalensis
Abstract
:1. Introduction
2. Results
2.1. Obtention of the Integrated Map of Large Metacentric Chromosome 1 of S. senegalensis
2.2. Synteny Analysis
2.3. Repetitive Sequences
3. Discussion
4. Materials and Methods
4.1. BAC Clones
4.2. Chromosome Preparation and Double BAC-FISH
4.3. Sequencing and Bioinformatic Analysis
4.4. Repetitive Elements Analysis
4.5. Synteny Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Name of BAC | Gene Annotation | References |
---|---|---|
36D3 | mcr4 | [16] |
5K5 | H1, H2a, H2b, H3, H4, slc1a3a, rx2, calr, eps15l1, klf2, ap1m1, tpm4, rab8a, cib3 | [16,23] |
10L10 | rx2, calr, eps15l1, klf2 | [16] |
10K23 | calr, eps15l1, otos, apod, arhgap21, opn3 | [15] |
73B7 | otos, apod, arhgap21 | [16] |
52C17 | vps41, esco1, rp1, sox17, mrpl15, lypla1, rgs20, oprk1, chmp5, fastkd3, mlrn, myom1 | [16] |
53B20 | lrrc32, mplz1, rabep1, vps37d, casr, gpr149, plch1, grsf1, rufy3, slc4a4, npffr2, daglb, spag9, hbad, hbb, rhbdf1, slc48a1, slc26a9, aanat, foxo6, pcbp4, disp3, bmp1, kansl3, antxr1, gfpt1, nfu1, c9orf78, med22, aak1 | This work |
16E16 | dmrt2, dmrt3 | [15,16] |
48K7 | dmrt2, dmrt3, dmrt1, c9orf117, kank1, fbp1 | [16] |
56H24 | akap9, hcn4, pip5k1a, znf687b, aqp10, hax1, ubap2l, c1orf43, tuft1 | [16] |
12D22 | H1, H2a, H2b, H3, H4, tmem70, ankrd45 | [23] |
48P7 | cyp8b1, rock1, ups14, thoc1, aqp1, crhr2, myl3, pth1r | [16] |
13G1 | wac | [16] |
1C2 | nbea | [16] |
Genes | C. semilaevis | S. maximus | T. nigroviridis | G. aculeatus | X. maculatus | O. latipes | D. rerio | L. oculatus |
---|---|---|---|---|---|---|---|---|
gpr149 | 4 | 3 | 16 | I | 18 | 13 | 1 | LG14 |
plch1 | 4 | 3 | 16 | I | 18 | 13 | 18 | LG14 |
daglb | 8 | 18 | 2 | V | 10 | 19 | 12 | 1 |
spag9 | 8 | 18 | 2 | V | 10 | 19 | 12 | LG10 |
hbad | 8 | 18 | 3 | XI | 10 | 8 | 3 | LG13 |
hbb | 8 | 18 | 3 | XI | 10 | 19 | 3 | LG13 |
rhbdf1 | 17 | 18 | 3 | XI | 10 | 8 | 3 | LG13 |
slc48a1 | 10 | 11 | 11 | XII | 1 | 7 | 23 | LG4 |
slc26a9 | 1 | 11 | 9 | XII | 1 | 7 | 23 | LG3 |
aanat | 9 | 8 | 3 | XI | 16 | 8 | 3 | LG13 |
foxo6 | 13 | 7 | 1 | X | 13 | 11 | 19 | LG6 |
pcbp4 | 1 | 6 | 9 | XII | 1 | 7 | 22 | LG5 |
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Rodríguez, M.E.; Molina, B.; Merlo, M.A.; Arias-Pérez, A.; Portela-Bens, S.; García-Angulo, A.; Cross, I.; Liehr, T.; Rebordinos, L. Evolution of the Proto Sex-Chromosome in Solea senegalensis. Int. J. Mol. Sci. 2019, 20, 5111. https://doi.org/10.3390/ijms20205111
Rodríguez ME, Molina B, Merlo MA, Arias-Pérez A, Portela-Bens S, García-Angulo A, Cross I, Liehr T, Rebordinos L. Evolution of the Proto Sex-Chromosome in Solea senegalensis. International Journal of Molecular Sciences. 2019; 20(20):5111. https://doi.org/10.3390/ijms20205111
Chicago/Turabian StyleRodríguez, María Esther, Belén Molina, Manuel Alejandro Merlo, Alberto Arias-Pérez, Silvia Portela-Bens, Aglaya García-Angulo, Ismael Cross, Thomas Liehr, and Laureana Rebordinos. 2019. "Evolution of the Proto Sex-Chromosome in Solea senegalensis" International Journal of Molecular Sciences 20, no. 20: 5111. https://doi.org/10.3390/ijms20205111