Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery
Abstract
:1. Introduction
2. Results
2.1. Development and Characterization of Ce6-PCL-NEs
2.2. Cellular Uptake of Ce6-PCL-NE and ROS Generation
2.3. Cytotoxicity Test of Ce6-PCL-NE with or without Laser Irradiation
2.4. In Vivo Biodistribution of Ce6-PCL-NE in 4T1 Tumor-Bearing Mice
2.5. Ex Vivo Analysis of Resected Organs and Tumor Tissues
2.6. Accumulation of Ce6-PCL-NE in Metastatic Tumor Tissues
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparation of Nanoemulsion
4.3. Characterization of Nanoparticles
4.4. Cellular Uptake and DCFDA Assay
4.5. Cell Viability with or without Laser Irradiation
4.6. In Vivo and Ex Vivo Imaging
4.7. Statistics
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
PEG-b-PCL | poly(ethylene glycol)-block-polycaprolactone |
PDT | photodynamic therapy |
PS | photosensitizer |
Ce6 | chlorin e6 |
NE | nanoemulsion |
NP | nanoparticle |
ROS | reactive oxygen species |
EPR | enhanced permeability and retention |
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Park, C.; Yoo, J.; Lee, D.; Jang, S.-y.; Kwon, S.; Koo, H. Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery. Int. J. Mol. Sci. 2019, 20, 3958. https://doi.org/10.3390/ijms20163958
Park C, Yoo J, Lee D, Jang S-y, Kwon S, Koo H. Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery. International Journal of Molecular Sciences. 2019; 20(16):3958. https://doi.org/10.3390/ijms20163958
Chicago/Turabian StylePark, Changhee, Jihye Yoo, Donghyun Lee, Seok-young Jang, Soonmin Kwon, and Heebeom Koo. 2019. "Chlorin e6-Loaded PEG-PCL Nanoemulsion for Photodynamic Therapy and In Vivo Drug Delivery" International Journal of Molecular Sciences 20, no. 16: 3958. https://doi.org/10.3390/ijms20163958