Comparison of Polydopamine-Coated Mesoporous Silica Nanorods and Spheres for the Delivery of Hydrophilic and Hydrophobic Anticancer Drugs
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of the Nanoparticles
2.2. In Vitro Evaluation of MSN@PDA as a Drug Delivery System
2.2.1. Cellular Uptake of MSN@PDA-COP Particles
2.2.2. Intracellular Distribution of DOX Delivered by MSN@PDA-COP Particles
2.2.3. Cytotoxicity of Empty and Drug-Loaded MSN@PDA-COP Particles
3. Materials and Methods
3.1. Polydopamine Coating of MSN
3.2. Characterization of MSN@PDA Particles
3.3. Drug Loading and Loading Degree Determination
3.4. Cell Cultures
3.5. Cellular Uptake of MSN@PDA
3.5.1. Confocal Microscopy
3.5.2. Fluorescent Activated Cell Sorting
3.6. Intracellular Distribution of DOX-Loaded MSN@PDA Particles
3.7. Cytotoxicity
3.7.1. WST-1 for DOX-Loaded Particles
3.7.2. Crystal Violet for DOX-Loaded Particles
3.7.3. Crystal Violet for FTY720-Loaded Particles
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
MSN | Mesoporous silica nanoparticles |
PDA | Polydopamine |
DOX | Doxorubicin |
FTY720 | Fingolimod |
MSN-NH2 | Amino-functionalized MSN |
MDA-MB-231 | Human breast cancer cells |
ML-1 | Human follicular thyroid cancer cells |
FACS | Fluorescence Activated Cell Sorting |
S-MSN | Spherical shaped MSN |
R-MSN | Rod shaped MSN |
PEG-PEI | Polyethylene glycol-Polyethylenimine |
DLS | Dynamic Light Scattering |
TEM | Transmission Electron Microscopy |
WST | Water Soluble Tetrazolium salt |
PI | Propidium Iodide |
FITC | Fluorescein isothiocyanate |
HEPES | 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid |
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Particle Type | Z-Average Size (nm) (Measured in DI Water) | Polydispersity Index (PdI) | Zeta Potential (mV) (Measured in HEPES Buffer, 25mM, pH 7.4) |
---|---|---|---|
S-MSN | 181.7 ± 1.45 | 0.191 | 15.3 |
Rod-MSN | 248.73 ± 3.90 | 0.087 | −20.83 ± 1.05 |
S-MSN@PDA | 191.77 ± 5.15 | 0.269 | −13.43 ± 0.31 |
Rod-MSN@PDA | 1678.00 ± 159.45 | 0.313 | −22.93 ± 0.96 |
S-MSN@PDA-COP | 158.80 ± 1.32 | 0.198 | 3.91 ± 0.49 |
Rod-MSN@PDA-COP | 219.60 ± 4.92 | 0.097 | 5.74 ± 0.39 |
Particle Type | Concentration (µg/mL) | Uptake by Cells (MFI) |
---|---|---|
S-MSN@PDA | 5 | 30 |
10 | 40 | |
25 | 70 | |
50 | 150 | |
R-MSN@PDA | 5 | 40 |
10 | 60 | |
25 | 165 |
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Pada, A.-K.; Desai, D.; Sun, K.; Prakirth Govardhanam, N.; Törnquist, K.; Zhang, J.; Rosenholm, J.M. Comparison of Polydopamine-Coated Mesoporous Silica Nanorods and Spheres for the Delivery of Hydrophilic and Hydrophobic Anticancer Drugs. Int. J. Mol. Sci. 2019, 20, 3408. https://doi.org/10.3390/ijms20143408
Pada A-K, Desai D, Sun K, Prakirth Govardhanam N, Törnquist K, Zhang J, Rosenholm JM. Comparison of Polydopamine-Coated Mesoporous Silica Nanorods and Spheres for the Delivery of Hydrophilic and Hydrophobic Anticancer Drugs. International Journal of Molecular Sciences. 2019; 20(14):3408. https://doi.org/10.3390/ijms20143408
Chicago/Turabian StylePada, Anna-Karin, Diti Desai, Kaiyao Sun, Narayana Prakirth Govardhanam, Kid Törnquist, Jixi Zhang, and Jessica M. Rosenholm. 2019. "Comparison of Polydopamine-Coated Mesoporous Silica Nanorods and Spheres for the Delivery of Hydrophilic and Hydrophobic Anticancer Drugs" International Journal of Molecular Sciences 20, no. 14: 3408. https://doi.org/10.3390/ijms20143408