Next Article in Journal
A Tiny Change Makes a Big Difference in the Anti-Parasitic Activities of an HDAC Inhibitor
Next Article in Special Issue
Adaptable Model Parameters in Non-Invasive Prenatal Testing Lead to More Stable Predictions
Previous Article in Journal
Towards Exploitation of Adaptive Traits for Climate-Resilient Smart Pulses
Previous Article in Special Issue
Cell-Free, Embryo-Specific sncRNA as a Molecular Biological Bridge between Patient Fertility and IVF Efficiency
Open AccessArticle

The Prediction of Gestational Hypertension, Preeclampsia and Fetal Growth Restriction via the First Trimester Screening of Plasma Exosomal C19MC microRNAs

1
Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, 10000 Prague, Czech Republic
2
Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, 14700 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2972; https://doi.org/10.3390/ijms20122972
Received: 29 March 2019 / Revised: 11 June 2019 / Accepted: 15 June 2019 / Published: 18 June 2019
(This article belongs to the Special Issue Cell-Free Nucleic Acids)
The aim of the study was to verify if quantification of placental specific C19MC microRNAs in plasma exosomes would be able to differentiate during the early stages of gestation between patients subsequently developing pregnancy-related complications and women with the normal course of gestation and if this differentiation would lead to the improvement of the diagnostical potential. The retrospective study on singleton Caucasian pregnancies was performed within 6/2011-2/2019. The case control study, nested in a cohort, involved women that later developed GH (n = 57), PE (n = 43), FGR (n = 63), and 102 controls. Maternal plasma exosome profiling was performed with the selection of C19MC microRNAs with diagnostical potential only (miR-516b-5p, miR-517-5p, miR-518b, miR-520a-5p, miR-520h, and miR-525-5p) using real-time RT-PCR. The down-regulation of miR-517-5p, miR-520a-5p, and miR-525-5p was observed in patients with later occurrence of GH and PE. Maternal plasma exosomal profiling of selected C19MC microRNAs also revealed a novel down-regulated biomarker during the first trimester of gestation (miR-520a-5p) for women destinated to develop FGR. First trimester circulating plasma exosomes possess the identical C19MC microRNA expression profile as placental tissues derived from patients with GH, PE and FGR after labor. The predictive accuracy of first trimester C19MC microRNA screening (miR-517-5p, miR-520a-5p, and miR-525-5p) for the diagnosis of GH and PE was significantly higher in the case of expression profiling of maternal plasma exosomes compared to expression profiling of the whole maternal plasma samples. View Full-Text
Keywords: C19MC microRNA; expression; exosomes; fetal growth restriction; gestational hypertension; plasma; prediction; preeclampsia; pregnancy-related complications; screening C19MC microRNA; expression; exosomes; fetal growth restriction; gestational hypertension; plasma; prediction; preeclampsia; pregnancy-related complications; screening
Show Figures

Graphical abstract

MDPI and ACS Style

Hromadnikova, I.; Dvorakova, L.; Kotlabova, K.; Krofta, L. The Prediction of Gestational Hypertension, Preeclampsia and Fetal Growth Restriction via the First Trimester Screening of Plasma Exosomal C19MC microRNAs. Int. J. Mol. Sci. 2019, 20, 2972. https://doi.org/10.3390/ijms20122972

AMA Style

Hromadnikova I, Dvorakova L, Kotlabova K, Krofta L. The Prediction of Gestational Hypertension, Preeclampsia and Fetal Growth Restriction via the First Trimester Screening of Plasma Exosomal C19MC microRNAs. International Journal of Molecular Sciences. 2019; 20(12):2972. https://doi.org/10.3390/ijms20122972

Chicago/Turabian Style

Hromadnikova, Ilona; Dvorakova, Lenka; Kotlabova, Katerina; Krofta, Ladislav. 2019. "The Prediction of Gestational Hypertension, Preeclampsia and Fetal Growth Restriction via the First Trimester Screening of Plasma Exosomal C19MC microRNAs" Int. J. Mol. Sci. 20, no. 12: 2972. https://doi.org/10.3390/ijms20122972

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop