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Article

Integrative Histologic and Bioinformatics Analysis of BIRC5/Survivin Expression in Oral Squamous Cell Carcinoma

by
Giuseppe Troiano
1,†,
Agostino Guida
2,†,
Gabriella Aquino
3,
Gerardo Botti
3,
Nunzia Simona Losito
3,
Silvana Papagerakis
4,
Maria Carmela Pedicillo
1,
Franco Ionna
2,
Francesco Longo
2,
Monica Cantile
3,*,
Antonio Pennella
1,
Lucio Lo Russo
1,
Giovanni Di Gioia
1,
Maria Addolorata Mariggiò
5,
Lorenzo Lo Muzio
1 and
Giuseppe Pannone
1
1
Department of Clinical and Experimental Medicine, University of Foggia, Via Rovelli 50, Foggia 71122, Italy
2
Maxillofacial and ENT Surgery Department, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione G. Pascale, IRCCS, Naples 80131, Italy
3
Pathology Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori, Fondazione G. Pascale, IRCCS, Naples 80131, Italy
4
Department of Surgery, Cancer Research Cluster, Room 4D10.2, Health Sciences Building, Saskatchewan University, Saskatoon, SKS7N5E5, Canada
5
Department of Biomedical Sciences and Human Oncology, University of Bari. Piazza Giulio Cesare 11, Bari 70124, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(9), 2664; https://doi.org/10.3390/ijms19092664
Submission received: 28 August 2018 / Accepted: 2 September 2018 / Published: 8 September 2018
(This article belongs to the Special Issue Head and Neck Cancers: Diagnostic, Prognostic and Predictive Biomarkers)
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Abstract

Survivin is a well-known protein involved in the inhibition of apoptosis in many different cancer types. The aim of this study was to perform an integrated bioinformatic and histologic analysis in order to study the expression and prognostic role of Survivin and its related gene BIRC5 in oral cancer. Publicly available databases were accessed via Gene Expression Omnibus and Oncomine, in addition raw data from The Cancer Genome Atlas (TCGA) were also obtained in order to analyze the rate of gene mutation, expression and methylation in patients with oral squamous cells carcinoma (OSCC). Immunohistochemistry (IHC) was also performed in order to evaluate the nuclear and cytoplasmic expression of Survivin and their correlation with cell proliferation in samples from OSCC patients. Results of this study revealed that Survivin is rarely mutated in OSCC samples and upregulated when compared to non-cancerous tissue. A negative correlation between the methylation of the island cg25986496 and BIRC5 mRNA expression was detected from TCGA data. IHC staining revealed that cytoplasmic (and not nuclear) expression of Survivin is associated with poor overall survival in OSCC patients, while the nuclear expression correlates with higher proliferation rate. In addition, data from TCGA database revealed that BIRC5 gene expression is an independent prognostic factor for OSCC patients.
Keywords: Survivin; BIRC5; immunohistochemistry; oral cancer; bioinformatic; TCGA Survivin; BIRC5; immunohistochemistry; oral cancer; bioinformatic; TCGA
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MDPI and ACS Style

Troiano, G.; Guida, A.; Aquino, G.; Botti, G.; Losito, N.S.; Papagerakis, S.; Pedicillo, M.C.; Ionna, F.; Longo, F.; Cantile, M.; et al. Integrative Histologic and Bioinformatics Analysis of BIRC5/Survivin Expression in Oral Squamous Cell Carcinoma. Int. J. Mol. Sci. 2018, 19, 2664. https://doi.org/10.3390/ijms19092664

AMA Style

Troiano G, Guida A, Aquino G, Botti G, Losito NS, Papagerakis S, Pedicillo MC, Ionna F, Longo F, Cantile M, et al. Integrative Histologic and Bioinformatics Analysis of BIRC5/Survivin Expression in Oral Squamous Cell Carcinoma. International Journal of Molecular Sciences. 2018; 19(9):2664. https://doi.org/10.3390/ijms19092664

Chicago/Turabian Style

Troiano, Giuseppe, Agostino Guida, Gabriella Aquino, Gerardo Botti, Nunzia Simona Losito, Silvana Papagerakis, Maria Carmela Pedicillo, Franco Ionna, Francesco Longo, Monica Cantile, and et al. 2018. "Integrative Histologic and Bioinformatics Analysis of BIRC5/Survivin Expression in Oral Squamous Cell Carcinoma" International Journal of Molecular Sciences 19, no. 9: 2664. https://doi.org/10.3390/ijms19092664

APA Style

Troiano, G., Guida, A., Aquino, G., Botti, G., Losito, N. S., Papagerakis, S., Pedicillo, M. C., Ionna, F., Longo, F., Cantile, M., Pennella, A., Lo Russo, L., Di Gioia, G., Mariggiò, M. A., Lo Muzio, L., & Pannone, G. (2018). Integrative Histologic and Bioinformatics Analysis of BIRC5/Survivin Expression in Oral Squamous Cell Carcinoma. International Journal of Molecular Sciences, 19(9), 2664. https://doi.org/10.3390/ijms19092664

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