Next Article in Journal
Molecular Analysis of Sensory Axon Branching Unraveled a cGMP-Dependent Signaling Cascade
Previous Article in Journal
Molecular Pharmacology of VEGF-A Isoforms: Binding and Signalling at VEGFR2
Open AccessArticle

Gastroprotective and Antioxidant Activity of Kalanchoe brasiliensis and Kalanchoe pinnata Leaf Juices against Indomethacin and Ethanol-Induced Gastric Lesions in Rats

1
Research Group on Bioactive Natural Products, Department of Pharmacy, Federal University of Rio Grande do Norte, 59012-570 Natal, Brazil
2
Department of Biophysics and Pharmacology, Biosciences Center, Federal University of Rio Grande do Norte, 59072-970 Natal, Brazil
3
Faculty of Health Sciences of Trairi, Federal University of Rio Grande do Norte, 59200-000 Santa Cruz, Brazil
4
Department of Morphology, Histology and Basic Pathology, Biosciences Center, Federal University of Rio Grande do Norte, 59072-970 Natal, Brazil
5
Department of Pharmacy, Federal University of Rio Grande do Norte, 59012-570 Natal, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(5), 1265; https://doi.org/10.3390/ijms19051265
Received: 7 March 2018 / Revised: 26 March 2018 / Accepted: 29 March 2018 / Published: 24 April 2018
(This article belongs to the Section Bioactives and Nutraceuticals)
Kalanchoe brasiliensis and Kalanchoe pinnata are used interchangeably in traditional medicine for treating peptic ulcers and inflammatory problems. In this context, this study aims to characterize the chemical constituents and evaluate the gastroprotective activity of the leaf juices of the two species in acute gastric lesions models. Thin Layer Chromatography (TLC) and Ultra High Performance Liquid Chromatography coupled to Mass Spectrometer (UHPLC-MS) were performed for chemical characterization. Wistar rats were pre-treated orally with leaf juices (125, 250 and 500 mg/kg) or ranitidine (50 mg/kg). The peaks observed in the chromatogram of K. brasiliensis showed similar mass spectra to flavonoid glycosides derived from patuletin and eupafolin, while K. pinnata showed mass spectra similar to compounds derived from quercetin, patuletin, eupafolin and kaempferol. K. brasiliensis at all doses and K. pinnata at doses of 250 mg/kg and 500 mg/kg significantly reduced the lesions in the ethanol induction model. In the indomethacin induction model, both species showed significant results at doses of 250 and 500 mg/kg. Also, the pre-treatment with leaf juices increased the antioxidant defense system, glutathione (GSH), whereas malondialdehyde (MDA), myeloperoxidase (MPO), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels were significantly decreased. Treatment with leaf juices led to the upregulation of zone occludes-1 (ZO-1) and the downregulation of inducible nitric oxide synthase (iNOS) and factor nuclear-κβ transcription (NF-κB-p65), while also showing a cytoprotective effect and maintaining mucus production. These findings show that the leaf juices of the two species showed gastroprotective effects on ethanol and gastric indomethacin injury which were a consequence of gastric inflammation suppression, antioxidant activity and the maintenance of cytoprotective defenses and mucosal structure architecture. View Full-Text
Keywords: Crassulaceae; coirama; peptic ulcers; flavonoids Crassulaceae; coirama; peptic ulcers; flavonoids
Show Figures

Figure 1

MDPI and ACS Style

De Araújo, E.R.D.; Guerra, G.C.B.; Araújo, D.F.S.; De Araújo, A.A.; Fernandes, J.M.; De Araújo Júnior, R.F.; Da Silva, V.C.; De Carvalho, T.G.; Ferreira, L.D.S.; Zucolotto, S.M. Gastroprotective and Antioxidant Activity of Kalanchoe brasiliensis and Kalanchoe pinnata Leaf Juices against Indomethacin and Ethanol-Induced Gastric Lesions in Rats. Int. J. Mol. Sci. 2018, 19, 1265.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop