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Open AccessArticle

Impact of Mutagens on DNA Replication in Barley Chromosomes

Department of Plant Anatomy and Cytology, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(4), 1070; https://doi.org/10.3390/ijms19041070
Received: 13 February 2018 / Revised: 26 March 2018 / Accepted: 31 March 2018 / Published: 3 April 2018
(This article belongs to the Special Issue DNA Replication Stress)
Replication errors that are caused by mutagens are critical for living cells. The aim of the study was to analyze the distribution of a DNA replication pattern on chromosomes of the H. vulgare ‘Start’ variety using pulse 5-ethynyl-2′-deoxyuridine (EdU) labeling, as well as its relationship to the DNA damage that is induced by mutagenic treatment with maleic hydrazide (MH) and γ ray. To the best of our knowledge, this is the first example of a study of the effects of mutagens on the DNA replication pattern in chromosomes, as well as the first to use EdU labeling for these purposes. The duration of the cell cycle of the Hordeum vulgare ‘Start’ variety was estimated for the first time, as well as the influence of MH and γ ray on it. The distribution of the signals of DNA replication along the chromosomes revealed relationships between DNA replication, the chromatin structure, and DNA damage. MH has a stronger impact on replication than γ ray. Application of EdU seems to be promising for precise analyses of cell cycle disturbances in the future, especially in plant species with small genomes. View Full-Text
Keywords: barley; chromosome; DNA replication pattern; EdU; mutagens barley; chromosome; DNA replication pattern; EdU; mutagens
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MDPI and ACS Style

Kwasniewska, J.; Zubrzycka, K.; Kus, A. Impact of Mutagens on DNA Replication in Barley Chromosomes. Int. J. Mol. Sci. 2018, 19, 1070.

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