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Article

Epidermal Growth Factor, through Alleviating Oxidative Stress, Protect IPEC-J2 Cells from Lipopolysaccharides-Induced Apoptosis

1
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
2
Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(3), 848; https://doi.org/10.3390/ijms19030848
Received: 21 January 2018 / Revised: 1 March 2018 / Accepted: 8 March 2018 / Published: 14 March 2018
(This article belongs to the Special Issue Free Radicals and Oxidants in Pathogenesis)
The epidermal growth factor (EGF) has been widely used for protection of stress-induced intestinal mucosa dysfunction. However, whether EGF would alleviate oxidative injury and reduce apoptosis in porcine intestine is not yet known. Therefore, the aim of this study was to investigate the effect of EGF on lipopolysaccharides (LPS)-induced induction of oxidative stress and ensuing apoptosis in the porcine intestinal epithelial cell line, IPEC-J2. The present study showed that EGF significantly increased cell viability and decreased the LPS-induced induction of apoptosis, dehydrogenase (LDH) release and malonaldehyde (MDA) production. EGF also (i) decreased expression of the pro-apoptotic genes Fas, Bax, Cascase-3, Cascase-8, Cascase-9, and proteins such as P53, Fas, Bax, Caspase3; (ii) increased antiapoptotic protein B-cell lymphoma 2 (Bcl2) expression; (iii) increased mRNA levels of the nuclear factor erythroid 2-related factor 2 (Nrf2) related genes Nrf2, manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GSH-Px), heme oxygenase (HO-1) and quinone oxidoreductase (NQO1); (iv) protein level of Nrf2-realeted proteins Nrf2, HO-1, NQO1; and (v) total antioxidant capacity (T-AOC), CAT, SOD, GSH-Px concentrations. Collectively, our results indicated that EGF enhanced Nrf2 protein expression, and upregulated the expression of phase II metabolizing enzymes (such as HO-1 and NQO1) and antioxidative enzymes (SOD, CAT and GSH-Px) to alleviate oxidative injury, and then protect IPEC-J2 cells from apoptosis induced by LPS. View Full-Text
Keywords: antioxidant; apoptosis; epidermal growth factor; lipopolysaccharides (LPS); oxidative stress antioxidant; apoptosis; epidermal growth factor; lipopolysaccharides (LPS); oxidative stress
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MDPI and ACS Style

Tang, X.; Liu, B.; Wang, X.; Yu, Q.; Fang, R. Epidermal Growth Factor, through Alleviating Oxidative Stress, Protect IPEC-J2 Cells from Lipopolysaccharides-Induced Apoptosis. Int. J. Mol. Sci. 2018, 19, 848. https://doi.org/10.3390/ijms19030848

AMA Style

Tang X, Liu B, Wang X, Yu Q, Fang R. Epidermal Growth Factor, through Alleviating Oxidative Stress, Protect IPEC-J2 Cells from Lipopolysaccharides-Induced Apoptosis. International Journal of Molecular Sciences. 2018; 19(3):848. https://doi.org/10.3390/ijms19030848

Chicago/Turabian Style

Tang, Xiaopeng, Bo Liu, Xiangrong Wang, Qifang Yu, and Rejun Fang. 2018. "Epidermal Growth Factor, through Alleviating Oxidative Stress, Protect IPEC-J2 Cells from Lipopolysaccharides-Induced Apoptosis" International Journal of Molecular Sciences 19, no. 3: 848. https://doi.org/10.3390/ijms19030848

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