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Int. J. Mol. Sci. 2018, 19(3), 761; https://doi.org/10.3390/ijms19030761

A Neutrophil Proteomic Signature in Surgical Trauma Wounds

1
Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis, Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany
2
Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, Greifswald University, Medical Center Ferdinand-Sauerbruch-Str., 17475 Greifswald, Germany
*
Author to whom correspondence should be addressed.
Received: 31 December 2017 / Revised: 1 March 2018 / Accepted: 2 March 2018 / Published: 7 March 2018
(This article belongs to the Special Issue New Innovations in Wound Healing and Repair)
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Abstract

Non-healing wounds continue to be a clinical challenge for patients and medical staff. These wounds have a heterogeneous etiology, including diabetes and surgical trauma wounds. It is therefore important to decipher molecular signatures that reflect the macroscopic process of wound healing. To this end, we collected wound sponge dressings routinely used in vacuum assisted therapy after surgical trauma to generate wound-derived protein profiles via global mass spectrometry. We confidently identified 311 proteins in exudates. Among them were expected targets belonging to the immunoglobulin superfamily, complement, and skin-derived proteins, such as keratins. Next to several S100 proteins, chaperones, heat shock proteins, and immune modulators, the exudates presented a number of redox proteins as well as a discrete neutrophil proteomic signature, including for example cathepsin G, elastase, myeloperoxidase, CD66c, and lipocalin 2. We mapped over 200 post-translational modifications (PTMs; cysteine/methionine oxidation, tyrosine nitration, cysteine trioxidation) to the proteomic profile, for example, in peroxiredoxin 1. Investigating manually collected exudates, we confirmed presence of neutrophils and their products, such as microparticles and fragments containing myeloperoxidase and DNA. These data confirmed known and identified less known wound proteins and their PTMs, which may serve as resource for future studies on human wound healing. View Full-Text
Keywords: chaperones; damage-associated molecular patterns; heat-shock proteins; mass spectrometry; matrix metalloproteinase; peptidases; post-translational modifications; redox regulation chaperones; damage-associated molecular patterns; heat-shock proteins; mass spectrometry; matrix metalloproteinase; peptidases; post-translational modifications; redox regulation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Bekeschus, S.; Lackmann, J.-W.; Gümbel, D.; Napp, M.; Schmidt, A.; Wende, K. A Neutrophil Proteomic Signature in Surgical Trauma Wounds. Int. J. Mol. Sci. 2018, 19, 761.

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