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Open AccessArticle

Development and Characterisation of a Human Chronic Skin Wound Cell Line—Towards an Alternative for Animal Experimentation

1
Stem Cells, Wound Repair & Regeneration Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff CF14 4XY, UK
2
Division of Cancer and Genetics, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
3
Division of Medical Genetics, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
4
Advanced Therapies Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff CF14 4XY, UK
*
Author to whom correspondence should be addressed.
Current address: Centre for Cutaneous Research, Blizard Institute, Barts and the London School of Medicine, QMUL, Blizard Building, 4 Newark Street, London E1 2AT, UK.
Current address: School of Life & Health Sciences, Aston University, Birmingham B4 7ET, UK.
§
Current address: School of Natural and Environmental Sciences, Devonshire Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Int. J. Mol. Sci. 2018, 19(4), 1001; https://doi.org/10.3390/ijms19041001
Received: 21 February 2018 / Revised: 21 March 2018 / Accepted: 23 March 2018 / Published: 27 March 2018
(This article belongs to the Special Issue New Innovations in Wound Healing and Repair)
Background: Chronic skin wounds are a growing financial burden for healthcare providers, causing discomfort/immobility to patients. Whilst animal chronic wound models have been developed to allow for mechanistic studies and to develop/test potential therapies, such systems are not good representations of the human chronic wound state. As an alternative, human chronic wound fibroblasts (CWFs) have permitted an insight into the dysfunctional cellular mechanisms that are associated with these wounds. However, such cells strains have a limited replicative lifespan and therefore a limited reproducibility/usefulness. Objectives: To develop/characterise immortalised cell lines of CWF and patient-matched normal fibroblasts (NFs). Methods and Results: Immortalisation with human telomerase resulted in both CWF and NF proliferating well beyond their replicative senescence end-point (respective cell strains senesced as normal). Gene expression analysis demonstrated that, whilst proliferation-associated genes were up-regulated in the cell lines (as would be expected), the immortalisation process did not significantly affect the disease-specific genotype. Immortalised CWF (as compared to NF) also retained a distinct impairment in their wound repopulation potential (in line with CWF cell strains). Conclusions: These novel CWF cell lines are a credible animal alternative and could be a valuable research tool for understanding both the aetiology of chronic skin wounds and for therapeutic pre-screening. View Full-Text
Keywords: wound healing; fibroblasts; chronic wound; microarray; animal alternative wound healing; fibroblasts; chronic wound; microarray; animal alternative
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MDPI and ACS Style

Caley, M.; Wall, I.B.; Peake, M.; Kipling, D.; Giles, P.; Thomas, D.W.; Stephens, P. Development and Characterisation of a Human Chronic Skin Wound Cell Line—Towards an Alternative for Animal Experimentation. Int. J. Mol. Sci. 2018, 19, 1001.

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