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Int. J. Mol. Sci. 2018, 19(3), 760; https://doi.org/10.3390/ijms19030760

Antimelanogenic Effect of an Oroxylum indicum Seed Extract by Suppression of MITF Expression through Activation of MAPK Signaling Protein

1
Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea
2
Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Korea
3
MR Innovation Co., Ltd., KNU Technopark, Daegu 41566, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 November 2017 / Revised: 13 February 2018 / Accepted: 14 February 2018 / Published: 7 March 2018
(This article belongs to the Special Issue Melanins and Melanogenesis: From Nature to Applications)
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Abstract

In this study, the antimelanogenic effect of an ethyl acetate fraction of Oroxylum indicum Vent. seeds (OISEA) and its underlying mechanisms in melan-a cells were investigated. Antimelanogenesis activity was confirmed by assessing inhibition of tyrosinase activity and melanin content in the cells. Both transcriptional and translational expression of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase related protein-1 and 2 (TYRP-1 and TYRP-2), were also examined. The results depicted that pretreatment of OISEA significantly inhibits not only tyrosinase activity, but melanin production and intracellular tyrosinase activity. By repressing the expression of tyrosinase, TYRP-1, TYRP-2, and MITF, OISEA interrupted melanin production. Additionally, OISEA interfered with the phosphorylation of p38, extracellular signal-regulated kinase 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK), with the reversal of OISEA-induced melanogenesis inhibition after treatment with the specific inhibitors SB239063, U0126, and SP600125. Overall, these results suggest that OISEA can stimulate p38, ERK1/2, JNK phosphorylation, and subsequent suppression of melanin, leading to the inhibition of melanogenic enzymes and melanin production, possibly owing to the presence of polyphenolic compounds. View Full-Text
Keywords: antimelanogenic; Oroxylum indicum; mitogen-activated protein kinase (MAPK); microphthalmia-associated transcription factor (MITF) antimelanogenic; Oroxylum indicum; mitogen-activated protein kinase (MAPK); microphthalmia-associated transcription factor (MITF)
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Zhao, P.; Alam, M.B.; An, H.; Choi, H.-J.; Cha, Y.H.; Yoo, C.-Y.; Kim, H.-H.; Lee, S.-H. Antimelanogenic Effect of an Oroxylum indicum Seed Extract by Suppression of MITF Expression through Activation of MAPK Signaling Protein. Int. J. Mol. Sci. 2018, 19, 760.

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