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Int. J. Mol. Sci. 2018, 19(3), 759; https://doi.org/10.3390/ijms19030759

Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma

1
Facility of Bio-Proteomics, Immunopathology and Cancer Biomarkers, CRO Aviano National Cancer Institute, 33081 Aviano, Italy
2
Gastroenterology, CRO Aviano National Cancer Institute, 33081 Aviano, Italy
3
Medical Oncology, CRO Aviano National Cancer Institute, 33081 Aviano, Italy
4
Immunopathology and Cancer Biomarkers, CRO Aviano National Cancer Institute, 33081 Aviano, Italy
5
Pathology, CRO Aviano National Cancer Institute, 33081 Aviano, Italy
*
Author to whom correspondence should be addressed.
Received: 22 January 2018 / Revised: 27 February 2018 / Accepted: 5 March 2018 / Published: 7 March 2018
(This article belongs to the Special Issue Molecular Features Distinguishing Gastric Cancer Subtypes)
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Abstract

Elevated plasma fibrinogen levels and tumor progression in patients with gastric cancer (GC) have been largely reported. However, distinct fibrinogen chains and domains have different effects on coagulation, inflammation, and angiogenesis. The aim of this study was to characterize fibrinogen β chain (FGB) in GC tissues. Retrospectively we analyzed the data of matched pairs of normal (N) and malignant tissues (T) of 28 consecutive patients with GC at diagnosis by combining one- and two-dimensional electrophoresis (1DE and 2DE) with immunoblotting and mass spectrometry together with two-dimensional difference in gel electrophoresis (2D-DIGE). 1DE showed bands of the intact FGB at 50 kDa and the cleaved forms containing the fragment D at ~37–40 kDa, which corresponded to 19 spots in 2DE. In particular, spot 402 at ~50 kDa and spots 526 and 548 at ~37 kDa were of interest by showing an increased expression in tumor tissues. A higher content of spot 402 was associated with stomach antrum, while spots 526 and 548 amounts correlated with corpus and high platelet count (>208 × 109/L). The quantification of FGB and cleaved products may help to further characterize the interconnections between GC and platelet/coagulation pathways. View Full-Text
Keywords: DIGE; comparative proteomics; gastric cancer; fibrinogen β chain; FGB; coagulation; platelets; biomarker DIGE; comparative proteomics; gastric cancer; fibrinogen β chain; FGB; coagulation; platelets; biomarker
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Repetto, O.; Maiero, S.; Magris, R.; Miolo, G.; Cozzi, M.R.; Steffan, A.; Canzonieri, V.; Cannizzaro, R.; De Re, V. Quantitative Proteomic Approach Targeted to Fibrinogen β Chain in Tissue Gastric Carcinoma. Int. J. Mol. Sci. 2018, 19, 759.

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