Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill
Abstract
:1. Introduction
2. Results
2.1. Leaf Relative Water Content (LRWC), Rate of Water Loss, Electrolyte Leakage, and Thiobarbituric Acid Reactive Substances (TBARS)
2.2. Hydrogen Peroxide (H2O2) and Superoxide (O2•−) Content
2.3. Proline (Pro) and Glycine Betaine (GB) Content
2.4. Activities of Antioxidant Enzymes
2.5. Activity of O-Acetylserine (Thiol) Lyase (OAS-TL) Enzyme and Cysteine (Cys) Content
2.6. Activities of LCD and DCD Enzymes and H2S Content
2.7. Photosynthetic Pigments and Carbonic Anhydrase (CA) Activity
3. Discussion
4. Materials and Methods
4.1. Plant Culture and Treatments
4.2. Estimation of Leaf Relative Water Content (LRWC), Electrolyte Leakage, and Rate of Water Loss
4.3. Detection of Hydrogen Peroxide (H2O2) and Superoxide (O2•−) in Roots
4.4. Estimation of H2O2 and O2•− Content
4.5. Analysis of Lipid Peroxidation
4.6. Determination of Proline (Pro) and Glycine Betaine (GB) Content
4.7. Assay of Antioxidant Enzymes
4.8. Determination of OAS-TL Enzyme Activity and Cys Content
4.9. Measurement of LCD and DCD Enzyme Activities and H2S Content
4.10. Estimation of Photosynthetic Pigments and Carbonic Anhydrase (CA) Activity
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Treatments | Parameters | |||||
---|---|---|---|---|---|---|
Chl a (mg g−1 FW) | Chl b (mg g−1 FW) | Total Chl (mg g−1 FW) | Chl a/b | Total carotenoids (mg g−1 FW) | CA activity (μM CO2 kg−1 leaf FW s−1) | |
Control | 1.84 ± 0.04 a | 0.96 ± 0.10 b | 2.80 ± 0.09 ab | 1.92 ± 0.29 cd | 3.68 ± 0.19 de | 276.40 ± 11.39 d |
DS | 1.37 ± 0.06 d | 0.51 ± 0.04 d | 1.88 ± 0.07 d | 2.69 ± 0.11 b | 4.15 ± 0.06 b | 321.58 ± 3.96 bc |
NaHS | 1.82 ± 0.06 ab | 1.07 ± 0.07 a | 2.89 ± 0.06 a | 1.70 ± 0.09 e | 3.82 ± 0.08 bc | 336.73 ± 4.96 b |
NaHS + DS | 1.77 ± 0.03 ac | 0.85 ± 0.10 c | 2.62 ± 0.08 ac | 2.08 ± 0.24 c | 4.78 ± 0.11 a | 371.27 ± 7.14 a |
HT + NaHS + DS | 1.25 ± 0.03 de | 0.25 ± 0.09 e | 1.50 ± 0.10 e | 5.00 ± 0.15 a | 3.76 ± 0.08 bd | 266.49 ± 15.22 de |
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Khan, M.N.; AlZuaibr, F.M.; Al-Huqail, A.A.; Siddiqui, M.H.; M. Ali, H.; Al-Muwayhi, M.A.; Al-Haque, H.N. Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill. Int. J. Mol. Sci. 2018, 19, 3981. https://doi.org/10.3390/ijms19123981
Khan MN, AlZuaibr FM, Al-Huqail AA, Siddiqui MH, M. Ali H, Al-Muwayhi MA, Al-Haque HN. Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill. International Journal of Molecular Sciences. 2018; 19(12):3981. https://doi.org/10.3390/ijms19123981
Chicago/Turabian StyleKhan, M. Nasir, Fahad M. AlZuaibr, Asma A. Al-Huqail, Manzer H. Siddiqui, Hayssam M. Ali, Mohammed A. Al-Muwayhi, and Hafiz N. Al-Haque. 2018. "Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill" International Journal of Molecular Sciences 19, no. 12: 3981. https://doi.org/10.3390/ijms19123981