Biocompatibility and Biological Efficiency of Inorganic Calcium Filled Bacterial Cellulose Based Hydrogel Scaffolds for Bone Bioengineering
Abstract
:1. Introduction
2. Results
2.1. Cell Viability and Biocompatibility Study
2.2. Genotoxic Potential of the Studied BC Based Inorganic Calcium Filled Hydrogel Scaffolds
2.3. Study on Apoptosis/Necrosis
2.4. SEM Analysis
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Synthesis and Preparation of Homogenous Suspension of BC
4.3. Preparation of Inorganic Calcium Phosphate Filled BC Based Hydrogel Scaffold
4.4. Preparation of Biomineralized Inorganic Calcium Phosphate and Calcium Carbonate (CaCO3) Filled BC Based Hydrogel Scaffold
4.5. Cell cultures
4.6. Evaluation of Cell Viability and Proliferation
4.6.1. Sample Preparation
4.6.2. Indirect Experiments (IDE)
4.6.3. Direct Experiments (DE)
4.7. Study of DNA Damages
4.8. Apoptosis/Necrosis Study via Annexin V-FITC
4.9. SEM Analysis
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Index | PVP (g) | CMC (g) | BC (g) | PEG (g) | Agar (g) | Glycerin (mL) | β-TCP/HA (g) | Water (mL) |
---|---|---|---|---|---|---|---|---|
BC-PVP | 0.5 | 0.0 | 0.5 | 1 | 2 | 1 | 0.0/0.0 | 95 |
BC-CMC | 0.0 | 0.5 | 0.5 | 1 | 2 | 1 | 0.0/0.0 | 95 |
BC-PVP-β-TCP/HA | 0.5 | 0.0 | 0.5 | 1 | 2 | 1 | 0.2/0.8 | 94 |
BC-CMC-β-TCP/HA | 0.0 | 0.5 | 0.5 | 1 | 2 | 1 | 0.2/0.8 | 94 |
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Basu, P.; Saha, N.; Alexandrova, R.; Andonova-Lilova, B.; Georgieva, M.; Miloshev, G.; Saha, P. Biocompatibility and Biological Efficiency of Inorganic Calcium Filled Bacterial Cellulose Based Hydrogel Scaffolds for Bone Bioengineering. Int. J. Mol. Sci. 2018, 19, 3980. https://doi.org/10.3390/ijms19123980
Basu P, Saha N, Alexandrova R, Andonova-Lilova B, Georgieva M, Miloshev G, Saha P. Biocompatibility and Biological Efficiency of Inorganic Calcium Filled Bacterial Cellulose Based Hydrogel Scaffolds for Bone Bioengineering. International Journal of Molecular Sciences. 2018; 19(12):3980. https://doi.org/10.3390/ijms19123980
Chicago/Turabian StyleBasu, Probal, Nabanita Saha, Radostina Alexandrova, Boyka Andonova-Lilova, Milena Georgieva, George Miloshev, and Petr Saha. 2018. "Biocompatibility and Biological Efficiency of Inorganic Calcium Filled Bacterial Cellulose Based Hydrogel Scaffolds for Bone Bioengineering" International Journal of Molecular Sciences 19, no. 12: 3980. https://doi.org/10.3390/ijms19123980