Overexpression of the DEAD-Box RNA Helicase Gene AtRH17 Confers Tolerance to Salt Stress in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Isolation of AtRH17 via Activation Tagging
2.2. Phylogenetic Analysis of AtRH17
2.3. Subcellular Localization of AtRH17 in the Nucleus
2.4. Expression of AtRH17 Is Unaffected by Osmotic Stress Conditions
2.5. Expression of AtRH17 during Developmental Stages and in Organs
2.6. AtRH17 OXs Exhibit Tolerance to Salt Stresses, but Not to Mannitol and Freezing Stresses
2.7. Expression of ABA-Dependent and ABA-Independent Salt-Stress-Responsive Genes Does Not Alter, but is Lower in AtRH17 OXs under Salt Stress Conditions
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Plasmid Construction and Plant Transformation
4.3. Activation Tagging Line Screening and Plant Stress Treatments
4.4. PS II Activity (Fv/Fm) and Chlorophyll Content Measurement
4.5. Histochemical Staining of Superoxide Production
4.6. RNA Extraction and RT-PCR Analysis
4.7. Multiple Sequence Alignment and Phylogenetic Analysis
4.8. Subcellular Localization of the AtRH17-GFP Fusion Protein
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
ABA | Abscisic acid |
AT | Activation tagging |
CaMV | Cauliflower mosaic virus |
DAG | Days after germination |
LD | Long-day |
MS | Murashige and Skoog |
NBT | Nitro blue tetrazolium |
OXs | Overexpressing transgenic plants |
RH | RNA helicase |
ROS | Reactive oxygen species |
SD | Short-day |
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Nguyen, L.V.; Seok, H.-Y.; Woo, D.-H.; Lee, S.-Y.; Moon, Y.-H. Overexpression of the DEAD-Box RNA Helicase Gene AtRH17 Confers Tolerance to Salt Stress in Arabidopsis. Int. J. Mol. Sci. 2018, 19, 3777. https://doi.org/10.3390/ijms19123777
Nguyen LV, Seok H-Y, Woo D-H, Lee S-Y, Moon Y-H. Overexpression of the DEAD-Box RNA Helicase Gene AtRH17 Confers Tolerance to Salt Stress in Arabidopsis. International Journal of Molecular Sciences. 2018; 19(12):3777. https://doi.org/10.3390/ijms19123777
Chicago/Turabian StyleNguyen, Linh Vu, Hye-Yeon Seok, Dong-Hyuk Woo, Sun-Young Lee, and Yong-Hwan Moon. 2018. "Overexpression of the DEAD-Box RNA Helicase Gene AtRH17 Confers Tolerance to Salt Stress in Arabidopsis" International Journal of Molecular Sciences 19, no. 12: 3777. https://doi.org/10.3390/ijms19123777