Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin

Yu, Feifei; Qu, Bingliang; Lin, Dandan; Deng, Yuewen; Huang, Ronglian; Zhong, Zhiming

doi:10.3390/ijms19123700

Open AccessArticle

Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin

by

Feifei Yu

,

Bingliang Qu

^*,

Dandan Lin

,

Yuewen Deng

,

Ronglian Huang

and

Zhiming Zhong

Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, China

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2018, 19(12), 3700; https://doi.org/10.3390/ijms19123700

Submission received: 24 October 2018 / Revised: 15 November 2018 / Accepted: 17 November 2018 / Published: 22 November 2018

(This article belongs to the Special Issue Transcriptional Regulation: Molecules, Involved Mechanisms and Misregulation)

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Abstract

The paired-box 3 (Pax3) is a transcription factor and it plays an important part in melanin synthesis. In this study, a new Pax3 gene was identified from Pteria penguin (Röding, 1798) (P. penguin) by RACE-PCR (rapid-amplification of cDNA ends-polymerase chain reaction) and its effect on melanin synthesis was deliberated by RNA interference (RNAi). The cDNA of PpPax3 was 2250 bp long, containing an open reading fragment of 1365 bp encoding 455 amino acids. Amino acid alignment and phylogenetic tree showed PpPax3 shared the highest (69.2%) identity with Pax3 of Mizuhopecten yessoensis. Tissue expression profile showed that PpPax3 had the highest expression in mantle, a nacre-formation related tissue. The PpPax3 silencing significantly inhibited the expression of PpPax3, PpMitf, PpTyr and PpCdk2, genes involved in Tyr-mediated melanin synthesis, but had no effect on PpCreb2 and an increase effect on PpBcl2. Furthermore, the PpPax3 knockdown obviously decreased the tyrosinase activity, the total content of eumelanin and the proportion of PDCA (pyrrole-2,3-dicarboxylic acid) in eumelanin, consistent with influence of tyrosinase (Tyr) knockdown. These data indicated that PpPax3 played an important regulating role in melanin synthesis by Tyr pathway in P. penguin.

Keywords: Pax3; Pteria penguin (Röding, 1798); tyrosinase; melanin; RNA interference; liquid chromatograph-tandem mass spectrometer (LC-MS/MS)

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MDPI and ACS Style

Yu, F.; Qu, B.; Lin, D.; Deng, Y.; Huang, R.; Zhong, Z. Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin. Int. J. Mol. Sci. 2018, 19, 3700. https://doi.org/10.3390/ijms19123700

AMA Style

Yu F, Qu B, Lin D, Deng Y, Huang R, Zhong Z. Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin. International Journal of Molecular Sciences. 2018; 19(12):3700. https://doi.org/10.3390/ijms19123700

Chicago/Turabian Style

Yu, Feifei, Bingliang Qu, Dandan Lin, Yuewen Deng, Ronglian Huang, and Zhiming Zhong. 2018. "Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin" International Journal of Molecular Sciences 19, no. 12: 3700. https://doi.org/10.3390/ijms19123700

APA Style

Yu, F., Qu, B., Lin, D., Deng, Y., Huang, R., & Zhong, Z. (2018). Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin. International Journal of Molecular Sciences, 19(12), 3700. https://doi.org/10.3390/ijms19123700

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Pax3 Gene Regulated Melanin Synthesis by Tyrosinase Pathway in Pteria penguin

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