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Article

Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats

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Post-Graduation Program in Oral Science, Department of Dentistry, UFRN, Natal 59072-970, Brazil
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Post-Graduation Program in Pharmacology/Post-Graduation Program in Morphology, Department of Morphology, UFC, Fortaleza 60440-900, Brazil
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Post-Graduation Program in Pharmaceutical Science/Post-Graduation Program in Health Science, Department of Pharmacology, UFRN, Natal 59072-970, Brazil
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Post-Graduation Program in Pharmaceutical Science, UFRN, Department of Pharmacology, Natal 59072-970, Brazil
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Periodontics Section, School of Dentistry, University of California, UCLA, Los Angeles, CA 90095, USA
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Post-Graduation Program in Functional and Structural Biology/Post-Graduation Program Health Science, Department of Morphology, UFRN, Natal 59072-970, Brazil
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Post-Graduation Program Biological Science/Post-Graduation Program in RENORBIO, Department of Biophysics and Pharmacology, UFRN, Natal 59072-970, Brazil
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Post-Graduation Program Biological Science/Post-Graduation Program in Pharmaceutical Science, Department of Biophysics and Pharmacology, UFRN, Natal 59072-970, Brazil
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Post-Graduation Program Oral Science/Post-Graduation Program in Pharmaceutical Science, Department of Biophysics and Pharmacology, UFRN, Natal 59072-970, Brazil
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Departamento de Biofisica e Farmacologia, Av. Senador Salgado Filho, S/N, Campus Universitário, Lagoa Nova, UFRN, Natal 59072-970, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(11), 3488; https://doi.org/10.3390/ijms19113488
Received: 18 July 2018 / Revised: 11 August 2018 / Accepted: 30 August 2018 / Published: 6 November 2018
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Evidence shows that metformin is an antidiabetic drug, which can exert favorable anti-inflammatory effects and decreased bone loss. The development of nanoparticles for metformin might be useful for increased therapeutic efficacy. The aim of this study was to evaluate the effect of metformin hydrochloride-loaded Poly (d,l-Lactide-co-glycolide) (PLGA)/(MET-loaded PLGA) on a ligature-induced periodontitis model in diabetic rats. MET-loaded PLGA were characterized by mean diameter, particle size, polydispensity index, and entrapment efficiency. Maxillae were scanned using Microcomputed Tomography (µCT) and histopathological and immunohistochemical analysis. IL-1β and TNF-α levels were analyzed by ELISA immunoassay. Quantitative RT-PCR was used (AMPK, NF-κB p65, HMGB1, and TAK-1). The mean diameter of MET-loaded PLGA nanoparticles was in a range of 457.1 ± 48.9 nm (p < 0.05) with a polydispersity index of 0.285 (p < 0.05), Z potential of 8.16 ± 1.1 mV (p < 0.01), and entrapment efficiency (EE) of 66.7 ± 3.73. Treatment with MET-loaded PLGA 10 mg/kg showed low inflammatory cells, weak staining by RANKL, cathepsin K, OPG, and osteocalcin, and levels of IL-1β and TNF-α (p < 0.05), increased AMPK expression gene (p < 0.05) and decreased NF-κB p65, HMGB1, and TAK-1 (p < 0.05). It is concluded that MET-loaded PLGA decreased inflammation and bone loss in periodontitis in diabetic rats. View Full-Text
Keywords: nanoparticles; poly lactic-co-glycolic acid; metformin; periodontal disease; inflammation nanoparticles; poly lactic-co-glycolic acid; metformin; periodontal disease; inflammation
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MDPI and ACS Style

Pereira, A.d.S.B.F.; Brito, G.A.d.C.; Lima, M.L.d.S.; Silva Júnior, A.A.d.; Silva, E.d.S.; De Rezende, A.A.; Bortolin, R.H.; Galvan, M.; Pirih, F.Q.; Araújo Júnior, R.F.d.; Medeiros, C.A.C.X.d.; Guerra, G.C.B.; Araújo, A.A.d. Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats. Int. J. Mol. Sci. 2018, 19, 3488. https://doi.org/10.3390/ijms19113488

AMA Style

Pereira AdSBF, Brito GAdC, Lima MLdS, Silva Júnior AAd, Silva EdS, De Rezende AA, Bortolin RH, Galvan M, Pirih FQ, Araújo Júnior RFd, Medeiros CACXd, Guerra GCB, Araújo AAd. Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats. International Journal of Molecular Sciences. 2018; 19(11):3488. https://doi.org/10.3390/ijms19113488

Chicago/Turabian Style

Pereira, Aline d.S.B.F., Gerly A.d.C. Brito, Maria L.d.S. Lima, Arnóbio A.d. Silva Júnior, Emanuell d.S. Silva, Adriana A. De Rezende, Raul H. Bortolin, Maria Galvan, Flávia Q. Pirih, Raimundo F.d. Araújo Júnior, Caroline A.C.X.d. Medeiros, Gerlane C.B. Guerra, and Aurigena A.d. Araújo 2018. "Metformin Hydrochloride-Loaded PLGA Nanoparticle in Periodontal Disease Experimental Model Using Diabetic Rats" International Journal of Molecular Sciences 19, no. 11: 3488. https://doi.org/10.3390/ijms19113488

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