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Article

TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis

by
Chih-Chun Ke
1,2,†,
Ying-Hung Lin
3,†,
Ya-Yun Wang
4,
Ying-Yu Wu
3,
Mei-Feng Chen
5,
Wei-Chi Ku
6,
Han-Sun Chiang
3 and
Tsung-Hsuan Lai
6,7,8,*
1
PhD Program in Nutrition & Food Science, Fu Jen Catholic University, New Taipei City 24205, Taiwan
2
Department of Urology, En Chu Kong Hospital, New Taipei City 23702, Taiwan
3
Graduate Institute of Biomedical and Pharmaceutical Science, Fu-Jen Catholic University, New Taipei City 24205, Taiwan
4
Department of Chemistry, Fu Jen Catholic University, New Taipei City 24205, Taiwan
5
Bone and Joint Research Center, Chang Gung Memorial Hospital, Taoyuan County 33305, Taiwan
6
School of Medicine, Fu Jen Catholic University, New Taipei City 24205, Taiwan
7
Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei 10630, Taiwan
8
Institute of Systems Biology and Bioinformatics, National Central University, Jhongli City, Taoyuan County 32001, Taiwan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(11), 3292; https://doi.org/10.3390/ijms19113292
Submission received: 28 September 2018 / Revised: 20 October 2018 / Accepted: 21 October 2018 / Published: 23 October 2018
(This article belongs to the Special Issue Small GTPases)
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Abstract

Few papers have focused on small guanosine triphosphate (GTP)-binding proteins and their regulation during spermatogenesis. TBC1D21 genes (also known as male germ cell RAB GTPase-activating protein MGCRABGAP) are related to sterility, as determined through cDNA microarray testing of human testicular tissues exhibiting spermatogenic defects. TBC1D21 is a protein specifically expressed in the testes that exhibits specific localizations of elongating and elongated spermatids during mammalian spermiogenesis. Furthermore, through co-immunoprecipitation (co-IP) and nano liquid chromatography–tandem mass spectrometry (nano LC–MS/MS), Rap1 has been recognized as a potential TBC1D21 interactor. This study determined the possible roles of Rap1 and TBC1D21 during mammalian spermiogenesis. First, the binding ability between Rap1 and TBC1D21 was verified using co-IP. Second, the stronger signals of Rap1 expressed in elongating and elongated murine spermatids extracted from testicular sections, namely spermatogonia, spermatocytes, and round spermatids, were compared. Third, Rap1 and TBC1D21 exhibited similar localizations at postacrosomal regions of spermatids and at the midpieces of mature sperms, through isolated male germ cells. Fourth, the results of an activating Rap1 pull-down assay indicated that TBC1D21 overexpression inactivates Rap1 activity in cell models. In conclusion, TBC1D21 may interact with and potentially regulate Rap1 during murine spermatogenesis.
Keywords: Rap1; TBC1D21; spermatids; male infertility Rap1; TBC1D21; spermatids; male infertility
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MDPI and ACS Style

Ke, C.-C.; Lin, Y.-H.; Wang, Y.-Y.; Wu, Y.-Y.; Chen, M.-F.; Ku, W.-C.; Chiang, H.-S.; Lai, T.-H. TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis. Int. J. Mol. Sci. 2018, 19, 3292. https://doi.org/10.3390/ijms19113292

AMA Style

Ke C-C, Lin Y-H, Wang Y-Y, Wu Y-Y, Chen M-F, Ku W-C, Chiang H-S, Lai T-H. TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis. International Journal of Molecular Sciences. 2018; 19(11):3292. https://doi.org/10.3390/ijms19113292

Chicago/Turabian Style

Ke, Chih-Chun, Ying-Hung Lin, Ya-Yun Wang, Ying-Yu Wu, Mei-Feng Chen, Wei-Chi Ku, Han-Sun Chiang, and Tsung-Hsuan Lai. 2018. "TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis" International Journal of Molecular Sciences 19, no. 11: 3292. https://doi.org/10.3390/ijms19113292

APA Style

Ke, C.-C., Lin, Y.-H., Wang, Y.-Y., Wu, Y.-Y., Chen, M.-F., Ku, W.-C., Chiang, H.-S., & Lai, T.-H. (2018). TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis. International Journal of Molecular Sciences, 19(11), 3292. https://doi.org/10.3390/ijms19113292

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