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Article

Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells

1
Department of Biomedical Chemistry, College of Biomedical & Health Science, Konkuk University, Chungju 27478, Chungbuk, Korea
2
Department of Food Bioscience, College of Biomedical & Health Science, Konkuk University, Chungju 27478, Chungbuk, Korea
3
Department of Life Science, College of Science and Technology, Woosuk University, 66 Daehak-ro, Jincheon-eup, Chungcheongbuk-do 27841, Korea
4
Department of Research & Development Center, BSB korea Co., Ltd., 66 Daehak-ro, Jincheon-eup, Chungcheongbuk-do 27841, Korea
5
Diabetes and Bio-Research Center, Konkuk University, Chungju 27478, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(10), 3149; https://doi.org/10.3390/ijms19103149
Received: 6 August 2018 / Revised: 28 September 2018 / Accepted: 11 October 2018 / Published: 13 October 2018
(This article belongs to the Special Issue Melanins and Melanogenesis: From Nature to Applications)
Zerumbone (ZER), an active constituent of the Zingiberaceae family, has been shown to exhibit several biological activities, such as anti-inflammatory, anti-allergic, anti-microbial, and anti-cancer; however, it has not been studied for anti-melanogenic properties. In the present study, we demonstrate that ZER and Zingiber officinale (ZO) extract significantly attenuate melanin accumulation in α-melanocyte-stimulating hormone (α-MSH)-stimulated mouse melanogenic B16F10 cells. Further, to elucidate the molecular mechanism by which ZER suppresses melanin accumulation, we analyzed the expression of melanogenesis-associated transcription factor, microphthalmia-associated transcription factor (MITF), and its target genes, such as tyrosinase, tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (TYRP2), in B16F10 cells that are stimulated by α-MSH. Here, we found that ZER inhibits the MITF-mediated expression of melanogenic genes upon α-MSH stimulation. Additionally, cells treated with different concentrations of zerumbone and ZO showed increased extracellular signal-regulated kinases 1 and 2 (ERK1/2) phosphorylation, which are involved in the degradation mechanism of MITF. Pharmacological inhibition of ERK1/2 using U0126 sufficiently reversed the anti-melanogenic effect of ZER, suggesting that increased phosphorylation of ERK1/2 is required for its anti-melanogenic activity. Taken together, these results suggest that ZER and ZO extract can be used as active ingredients in skin-whitening cosmetics because of their anti-melanogenic effect. View Full-Text
Keywords: zerumbone; Zingiber officinale roscoe; melanogenesis; MITF; ERK1/2 zerumbone; Zingiber officinale roscoe; melanogenesis; MITF; ERK1/2
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MDPI and ACS Style

Oh, T.-I.; Jung, H.-J.; Lee, Y.-M.; Lee, S.; Kim, G.-H.; Kan, S.-Y.; Kang, H.; Oh, T.; Ko, H.M.; Kwak, K.-C.; Lim, J.-H. Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells. Int. J. Mol. Sci. 2018, 19, 3149. https://doi.org/10.3390/ijms19103149

AMA Style

Oh T-I, Jung H-J, Lee Y-M, Lee S, Kim G-H, Kan S-Y, Kang H, Oh T, Ko HM, Kwak K-C, Lim J-H. Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells. International Journal of Molecular Sciences. 2018; 19(10):3149. https://doi.org/10.3390/ijms19103149

Chicago/Turabian Style

Oh, Taek-In, Hye-Jeong Jung, Yoon-Mi Lee, Sujin Lee, Geon-Hee Kim, Sang-Yeon Kan, Hyeji Kang, Taerim Oh, Hyun M. Ko, Keun-Chang Kwak, and Ji-Hong Lim. 2018. "Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells" International Journal of Molecular Sciences 19, no. 10: 3149. https://doi.org/10.3390/ijms19103149

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