Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells
AbstractZerumbone (ZER), an active constituent of the Zingiberaceae family, has been shown to exhibit several biological activities, such as anti-inflammatory, anti-allergic, anti-microbial, and anti-cancer; however, it has not been studied for anti-melanogenic properties. In the present study, we demonstrate that ZER and Zingiber officinale (ZO) extract significantly attenuate melanin accumulation in α-melanocyte-stimulating hormone (α-MSH)-stimulated mouse melanogenic B16F10 cells. Further, to elucidate the molecular mechanism by which ZER suppresses melanin accumulation, we analyzed the expression of melanogenesis-associated transcription factor, microphthalmia-associated transcription factor (MITF), and its target genes, such as tyrosinase, tyrosinase-related protein 1 (TYRP1), and tyrosinase-related protein 2 (TYRP2), in B16F10 cells that are stimulated by α-MSH. Here, we found that ZER inhibits the MITF-mediated expression of melanogenic genes upon α-MSH stimulation. Additionally, cells treated with different concentrations of zerumbone and ZO showed increased extracellular signal-regulated kinases 1 and 2 (ERK1/2) phosphorylation, which are involved in the degradation mechanism of MITF. Pharmacological inhibition of ERK1/2 using U0126 sufficiently reversed the anti-melanogenic effect of ZER, suggesting that increased phosphorylation of ERK1/2 is required for its anti-melanogenic activity. Taken together, these results suggest that ZER and ZO extract can be used as active ingredients in skin-whitening cosmetics because of their anti-melanogenic effect. View Full-Text
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Oh, T.-I.; Jung, H.-J.; Lee, Y.-M.; Lee, S.; Kim, G.-H.; Kan, S.-Y.; Kang, H.; Oh, T.; Ko, H.M.; Kwak, K.-C.; Lim, J.-H. Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells. Int. J. Mol. Sci. 2018, 19, 3149.
Oh T-I, Jung H-J, Lee Y-M, Lee S, Kim G-H, Kan S-Y, Kang H, Oh T, Ko HM, Kwak K-C, Lim J-H. Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells. International Journal of Molecular Sciences. 2018; 19(10):3149.Chicago/Turabian Style
Oh, Taek-In; Jung, Hye-Jeong; Lee, Yoon-Mi; Lee, Sujin; Kim, Geon-Hee; Kan, Sang-Yeon; Kang, Hyeji; Oh, Taerim; Ko, Hyun M.; Kwak, Keun-Chang; Lim, Ji-Hong. 2018. "Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MSH-Induced Melanogenesis in B16F10 Cells." Int. J. Mol. Sci. 19, no. 10: 3149.
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