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Open AccessArticle

Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion

1
New Therapeutic Targets Laboratory (TargetsLab)—Oncology Unit, Department of Medical Sciences, Faculty of Medicine, University of Girona, Emili Grahit 77, 17003 Girona, Spain
2
Product, Process and Production Engineering Research Group (GREP), Department of Mechanical Engineering and Industrial Construction, University of Girona, Maria Aurèlia Capmany 61, 17003 Girona, Spain
3
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Pic de Peguera 15, 17003 Girona, Spain
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 3148; https://doi.org/10.3390/ijms19103148
Received: 10 September 2018 / Revised: 10 October 2018 / Accepted: 11 October 2018 / Published: 12 October 2018
(This article belongs to the Special Issue Cell Colonization in Scaffolds)
Breast cancer stem cells (BCSCs) are tumor-initiating cells responsible for metastasis and tumor reappearance, but their research is limited by the impossibility to cultivate them in a monolayer culture. Scaffolds are three-dimensional (3D) cell culture systems which avoid problems related with culturing BCSC. However, a standardized scaffold for enhancing a BCSC population is still an open issue. The main aim of this study is to establish a suitable poly (lactic acid) (PLA) scaffold which will produce BCSC enrichment, thus allowing them to be studied. Different 3D printing parameters were analyzed using Taguchi experimental design methods. Several PLA scaffold architectures were manufactured using a Fused Filament Fabrication (FFF) 3D printer. They were then evaluated by cell proliferation assay and the configurations with the highest growth rates were subjected to BCSC quantification by ALDH activity. The design SS1 (0.2 mm layer height, 70% infill density, Zigzag infill pattern, 45° infill direction, and 100% flow) obtained the highest proliferation rate and was capable of enhancing a ALDH+ cell population compared to 2D cell culture. In conclusion, the data obtained endorse the PLA porous scaffold as useful for culturing breast cancer cells in a microenvironment similar to in vivo and increasing the numbers of BCSCs. View Full-Text
Keywords: 3D printing; three-dimensional cell culture; scaffolds; PLA; TNBC; breast cancer stem cells 3D printing; three-dimensional cell culture; scaffolds; PLA; TNBC; breast cancer stem cells
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Polonio-Alcalá, E.; Rabionet, M.; Guerra, A.J.; Yeste, M.; Ciurana, J.; Puig, T. Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion. Int. J. Mol. Sci. 2018, 19, 3148.

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