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Open AccessArticle

Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

1
Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99202, USA
2
Tissue Imaging and Proteomics Laboratory, Washington State University, Pullman, WA 99164, USA
3
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(10), 2912; https://doi.org/10.3390/ijms19102912
Received: 16 September 2018 / Revised: 22 September 2018 / Accepted: 22 September 2018 / Published: 25 September 2018
(This article belongs to the Special Issue Extracellular Matrix in Development and Disease)
Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer. View Full-Text
Keywords: porcine; breast cancer; extracellular matrix; hydrogel; tissue matrix scaffold; scaffold; 3D culture porcine; breast cancer; extracellular matrix; hydrogel; tissue matrix scaffold; scaffold; 3D culture
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MDPI and ACS Style

Rijal, G.; Wang, J.; Yu, I.; Gang, D.R.; Chen, R.K.; Li, W. Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture. Int. J. Mol. Sci. 2018, 19, 2912.

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