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Effect of Clotting Duration and Temperature on BDNF Measurement in Human Serum

Centro Cardiologico Monzino, IRCCS, 20138 Milan, Italy
Department of Pharmacological and Biomolecular Sciences, University of Milan, 20133 Milan, Italy
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(9), 1987;
Received: 11 August 2017 / Revised: 29 August 2017 / Accepted: 2 September 2017 / Published: 15 September 2017
(This article belongs to the Special Issue Brain-Derived Neurotrophic Factor)
Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage. However, the influences of normothermic conditions (37 °C) and of clotting duration on BDNF levels in human serum have not been investigated yet. Here, we showed that time and temperature during serum preparation could be taken into consideration to assess the association and/or impact of BDNF levels in the occurrence of pathological conditions. View Full-Text
Keywords: BDNF; temperature; clotting duration; serum BDNF; temperature; clotting duration; serum
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Amadio, P.; Sandrini, L.; Ieraci, A.; Tremoli, E.; Barbieri, S.S. Effect of Clotting Duration and Temperature on BDNF Measurement in Human Serum. Int. J. Mol. Sci. 2017, 18, 1987.

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