Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro
Abstract
:1. Introduction
2. Results
2.1. Distribution of Stem Cells in Adult Human Dental Pulp
2.2. Parallel Multilineage Differentiation in DPC-Formed Spheroids under Osteogenic Medium
2.3. Neuronal Differentiation of DPC-Formed Spheroids
2.4. Promotion Effect of DPCs on Neurogenesis in Organotypic Hippocampal Slices
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. 3D Culture of Spheroids on Matrigel
4.3. Organotypic Hippocampus Slice Culture
4.4. Immunohistochemistry
4.5. Histological Analysis
4.6. Whole-Cell Patch Recording
4.7. Cell Viability Assay
4.8. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Xiao, L.; Ide, R.; Saiki, C.; Kumazawa, Y.; Okamura, H. Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro. Int. J. Mol. Sci. 2017, 18, 1745. https://doi.org/10.3390/ijms18081745
Xiao L, Ide R, Saiki C, Kumazawa Y, Okamura H. Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro. International Journal of Molecular Sciences. 2017; 18(8):1745. https://doi.org/10.3390/ijms18081745
Chicago/Turabian StyleXiao, Li, Ryoji Ide, Chikako Saiki, Yasuo Kumazawa, and Hisashi Okamura. 2017. "Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro" International Journal of Molecular Sciences 18, no. 8: 1745. https://doi.org/10.3390/ijms18081745