Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Lin, Abraham; Truong, Billy; Patel, Sohil; Kaushik, Nagendra; Choi, Eun Ha; Fridman, Gregory; Fridman, Alexander; Miller, Vandana

doi:10.3390/ijms18050966

Open AccessArticle

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

by

Abraham Lin

¹,

Billy Truong

¹,

Sohil Patel

¹,

Nagendra Kaushik

²,

Eun Ha Choi

²

,

Gregory Fridman

¹,

Alexander Fridman

¹ and

Vandana Miller

^1,*

¹

C. & J. Nyheim Plasma Institute, Drexel University, Philadelphia, PA 19104, USA

²

Plasma Bioscience Research Center, Kwangwoon University, Seoul 139791, Korea

^*

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2017, 18(5), 966; https://doi.org/10.3390/ijms18050966

Submission received: 1 March 2017 / Revised: 27 April 2017 / Accepted: 28 April 2017 / Published: 3 May 2017

(This article belongs to the Special Issue Tumor Targeting Therapy and Selective Killing)

Download

Browse Figures

Versions Notes

Abstract

A novel application for non-thermal plasma is the induction of immunogenic cancer cell death for cancer immunotherapy. Cells undergoing immunogenic death emit danger signals which facilitate anti-tumor immune responses. Although pathways leading to immunogenic cell death are not fully understood; oxidative stress is considered to be part of the underlying mechanism. Here; we studied the interaction between dielectric barrier discharge plasma and cancer cells for oxidative stress-mediated immunogenic cell death. We assessed changes to the intracellular oxidative environment after plasma treatment and correlated it to emission of two danger signals: surface-exposed calreticulin and secreted adenosine triphosphate. Plasma-generated reactive oxygen and charged species were recognized as the major effectors of immunogenic cell death. Chemical attenuators of intracellular reactive oxygen species successfully abrogated oxidative stress following plasma treatment and modulated the emission of surface-exposed calreticulin. Secreted danger signals from cells undergoing immunogenic death enhanced the anti-tumor activity of macrophages. This study demonstrated that plasma triggers immunogenic cell death through oxidative stress pathways and highlights its potential development for cancer immunotherapy.

Keywords: non-thermal plasma; cancer immunotherapy; immunogenic cell death; oxidative stress; calreticulin; adenosine triphosphate; plasma immunotherapy; nanosecond-pulsed dielectric barrier discharge

Graphical Abstract

Share and Cite

MDPI and ACS Style

Lin, A.; Truong, B.; Patel, S.; Kaushik, N.; Choi, E.H.; Fridman, G.; Fridman, A.; Miller, V. Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress. Int. J. Mol. Sci. 2017, 18, 966. https://doi.org/10.3390/ijms18050966

AMA Style

Lin A, Truong B, Patel S, Kaushik N, Choi EH, Fridman G, Fridman A, Miller V. Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress. International Journal of Molecular Sciences. 2017; 18(5):966. https://doi.org/10.3390/ijms18050966

Chicago/Turabian Style

Lin, Abraham, Billy Truong, Sohil Patel, Nagendra Kaushik, Eun Ha Choi, Gregory Fridman, Alexander Fridman, and Vandana Miller. 2017. "Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress" International Journal of Molecular Sciences 18, no. 5: 966. https://doi.org/10.3390/ijms18050966

APA Style

Lin, A., Truong, B., Patel, S., Kaushik, N., Choi, E. H., Fridman, G., Fridman, A., & Miller, V. (2017). Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress. International Journal of Molecular Sciences, 18(5), 966. https://doi.org/10.3390/ijms18050966

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI