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Int. J. Mol. Sci. 2017, 18(3), 540;

Expression of Autophagy-Related Proteins in Different Types of Thyroid Cancer

Department of Pathology, Yonsei University College of Medicine, Seoul 130-701, Korea
Author to whom correspondence should be addressed.
Academic Editor: Yogesh Kumar Vashist
Received: 3 January 2017 / Revised: 22 February 2017 / Accepted: 24 February 2017 / Published: 2 March 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Thyroid cancer is common type of malignant tumor in humans, and the autophagy status of such tumors may vary according to subtype. This study aimed to investigate the expression and implications of the major autophagy-related molecules light chain (LC) 3A, LC3B, p62, and BNIP-3 in human thyroid carcinoma. Tissue microarrays were constructed from 555 thyroid cancers (papillary thyroid carcinoma (PTC): 342; follicular carcinoma (FC): 112; medullary carcinoma (MC): 70; poorly differentiated carcinoma (PDC): 23; and anaplastic carcinoma (AC): 8) and 152 follicular adenomas (FAs). Expression of autophagy-related molecules (LC3A, LC3B, p62, and BNIP-3) was detected immunohistochemically, and the results were analyzed via comparison with clinicopathologic parameters. Tumoral LC3A and LC3B expressions were the highest in MC (p < 0.001), whereas stromal LC3A expression was higher in AC and PTC (p < 0.001). BNIP-3 expression was absent in MC and AC (p = 0.013). Tumoral LC3A, LC3B, and p62 expressions were higher in conventional type PTC, compared with those in the follicular variant. PTC with the BRAF V600E mutation exhibited higher expression of all autophagy-related proteins relative to PTC without this mutation (p < 0.05). BNIP-3 negativity was associated with capsular invasion in FC (p = 0.001), and p62 negativity was associated with lymph node metastasis in MC (p = 0.006). In a univariate analysis, LC3B negativity was associated with shorter disease-free survival in PTC with the BRAF V600E mutation (p = 0.024). We conclude that the expression of autophagy-related proteins differs according to thyroid cancer subtype. View Full-Text
Keywords: autophagy; subtype; thyroid cancer; stroma; pathology autophagy; subtype; thyroid cancer; stroma; pathology

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kim, H.M.; Kim, E.-S.; Koo, J.S. Expression of Autophagy-Related Proteins in Different Types of Thyroid Cancer. Int. J. Mol. Sci. 2017, 18, 540.

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