Next Article in Journal
Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats
Next Article in Special Issue
Predicting MicroRNA Biomarkers for Cancer Using Phylogenetic Tree and Microarray Analysis
Previous Article in Journal
RVMAB: Using the Relevance Vector Machine Model Combined with Average Blocks to Predict the Interactions of Proteins from Protein Sequences
Previous Article in Special Issue
MicroRNA in Metabolic Re-Programming and Their Role in Tumorigenesis
Article

MicroRNA-19b Downregulates Gap Junction Protein Alpha1 and Synergizes with MicroRNA-1 in Viral Myocarditis

1
Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
2
Department of Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
3
Key Laboratory of Viral Heart Diseases, Ministry of Public Health, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China
4
Department of Forensic Medicine, Soochow University, Suzhou 215123, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Y-h. Taguchi
Int. J. Mol. Sci. 2016, 17(5), 741; https://doi.org/10.3390/ijms17050741
Received: 29 March 2016 / Revised: 7 May 2016 / Accepted: 10 May 2016 / Published: 18 May 2016
(This article belongs to the Special Issue MicroRNA Regulation)
Viral myocarditis (VMC) is a life-threatening disease that leads to heart failure or cardiac arrhythmia. A large number of researches have revealed that mircroRNAs (miRNAs) participate in the pathological processes of VMC. We previously reported that miR-1 repressed the expression of gap junction protein α1 (GJA1) in VMC. In this study, miR-19b was found to be significantly upregulated using the microarray analysis in a mouse model of VMC, and overexpression of miR-19b led to irregular beating pattern in human cardiomyocytes derived from the induced pluripotent stem cells (hiPSCs-CMs). The upregulation of miR-19b was associated with decreased GJA1 in vivo. Furthermore, a miR-19b inhibitor increased, while its mimics suppressed the expression of GJA1 in HL-1 cells. When GJA1 was overexpressed, the miR-19b mimics-mediated irregular beating was reversed in hiPSCs-CMs. In addition, the effect of miR-19b on GJA1 was enhanced by miR-1 in a dose-dependent manner. These data suggest miR-19b contributes to irregular beating through regulation of GJA1 by cooperating with miR-1. Based on the present and our previous studies, it could be indicated that miR-19b and miR-1 might be critically involved in cardiac arrhythmia associated with VMC. View Full-Text
Keywords: viral myocarditis; microRNA; gap junction; miR-19b; miR-1 viral myocarditis; microRNA; gap junction; miR-19b; miR-1
Show Figures

Graphical abstract

MDPI and ACS Style

Lin, J.; Xue, A.; Li, L.; Li, B.; Li, Y.; Shen, Y.; Sun, N.; Chen, R.; Xu, H.; Zhao, Z. MicroRNA-19b Downregulates Gap Junction Protein Alpha1 and Synergizes with MicroRNA-1 in Viral Myocarditis. Int. J. Mol. Sci. 2016, 17, 741. https://doi.org/10.3390/ijms17050741

AMA Style

Lin J, Xue A, Li L, Li B, Li Y, Shen Y, Sun N, Chen R, Xu H, Zhao Z. MicroRNA-19b Downregulates Gap Junction Protein Alpha1 and Synergizes with MicroRNA-1 in Viral Myocarditis. International Journal of Molecular Sciences. 2016; 17(5):741. https://doi.org/10.3390/ijms17050741

Chicago/Turabian Style

Lin, Junyi, Aimin Xue, Liliang Li, Beixu Li, Yuhua Li, Yiwen Shen, Ning Sun, Ruizhen Chen, Hongfei Xu, and Ziqin Zhao. 2016. "MicroRNA-19b Downregulates Gap Junction Protein Alpha1 and Synergizes with MicroRNA-1 in Viral Myocarditis" International Journal of Molecular Sciences 17, no. 5: 741. https://doi.org/10.3390/ijms17050741

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop