Next Article in Journal
Heart Disease in Women: Unappreciated Challenges, GPER as a New Target
Next Article in Special Issue
Home-Processed Red Beetroot (Beta vulgaris L.) Products: Changes in Antioxidant Properties and Bioaccessibility
Previous Article in Journal
MicroRNA-19b Downregulates Gap Junction Protein Alpha1 and Synergizes with MicroRNA-1 in Viral Myocarditis
Previous Article in Special Issue
Supercritical Carbon Dioxide and Microwave-Assisted Extraction of Functional Lipophilic Compounds from Arthrospira platensis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 17
Issue 5
10.3390/ijms17050758
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats

by
Min Ji Bak
1,†,
Van-Long Truong
1,†,
Se-Yeon Ko
1,
Xuan Ngan Giang Nguyen
1,
Pajaree Ingkasupart
1,
Mira Jun
2,
Jin Young Shin
3 and
Woo-Sik Jeong
1,*
1
Department of Smart Food and Drug, College of Biomedical Science & Engineering, Inje University, Gimhae 50834, Korea
2
Department of Food Science and Nutrition, Dong-A University, Busan 49315, Korea
3
Chemistry and Environmental Science, City University of New York—Medgar Evers College, New York, NY 11225, USA
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2016, 17(5), 758; https://doi.org/10.3390/ijms17050758
Submission received: 12 April 2016 / Revised: 6 May 2016 / Accepted: 12 May 2016 / Published: 18 May 2016
(This article belongs to the Special Issue Macro- and Micro-nutrient Antioxidants)
Browse Figures
Versions Notes

Abstract

In the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of wild grape seed procyanidins (WGP) against oxidative stress damage in ethanol-treated HepG2 cell and Sprague-Dawley (SD)-rat models. In HepG2 cells, WGP not only diminished the ethanol (EtOH, 100 mM)-induced reactive oxygen species (ROS) formation and cytochrome P450 2E1 (CYP2E1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. Additionally, to investigate the hepatoprotective effect of WGP, rats were orally administered 10 or 50 mg/kg WGP once daily for seven days prior to the single oral administration of EtOH (6 g/kg). The results show that WGP administration decreased the EtOH-induced augment of the levels of serum aspartate transaminase and alanine transaminase as well as serum alcohol and acetaldehyde. WGP treatment upregulated the activities and protein levels of hepatic alcohol dehydrogenase, aldehyde dehydrogenase, and antioxidant enzymes but downregulated the protein expression level of liver CYP2E1 in EtOH-treated rats. Moreover, the decreased phosphorylation levels of mitogen activated protein kinases (MAPKs) by ethanol were induced in both HepG2 cell and rat models. Overall, pretreatment of WGP displayed the protective activity against EtOH-mediated toxicity through the regulation of antioxidant enzymes and alcohol metabolism systems via MAPKs pathways.
Keywords: procyanidins; alcohol; oxidative stress; hepatoprotection; CYP2E1; MAPKs procyanidins; alcohol; oxidative stress; hepatoprotection; CYP2E1; MAPKs
Graphical Abstract

Share and Cite

MDPI and ACS Style

Bak, M.J.; Truong, V.-L.; Ko, S.-Y.; Nguyen, X.N.G.; Ingkasupart, P.; Jun, M.; Shin, J.Y.; Jeong, W.-S. Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats. Int. J. Mol. Sci. 2016, 17, 758. https://doi.org/10.3390/ijms17050758

AMA Style

Bak MJ, Truong V-L, Ko S-Y, Nguyen XNG, Ingkasupart P, Jun M, Shin JY, Jeong W-S. Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats. International Journal of Molecular Sciences. 2016; 17(5):758. https://doi.org/10.3390/ijms17050758

Chicago/Turabian Style

Bak, Min Ji, Van-Long Truong, Se-Yeon Ko, Xuan Ngan Giang Nguyen, Pajaree Ingkasupart, Mira Jun, Jin Young Shin, and Woo-Sik Jeong. 2016. "Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats" International Journal of Molecular Sciences 17, no. 5: 758. https://doi.org/10.3390/ijms17050758

APA Style

Bak, M. J., Truong, V.-L., Ko, S.-Y., Nguyen, X. N. G., Ingkasupart, P., Jun, M., Shin, J. Y., & Jeong, W.-S. (2016). Antioxidant and Hepatoprotective Effects of Procyanidins from Wild Grape (Vitis amurensis) Seeds in Ethanol-Induced Cells and Rats. International Journal of Molecular Sciences, 17(5), 758. https://doi.org/10.3390/ijms17050758

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop