In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates towards Osteoblastic Cells (hFOB1.19)
Abstract
:1. Introduction
2. Results and Discussion
2.1. In Vitro Proliferation of the Six Hydrolysates towards hFOB1.19 Cells
Hydrolysates | Hydrolysis Times (h) | Other Conditions | DH (%) |
---|---|---|---|
CH1 | 1.5 | pH 6.0, 60 °C, papain of 2 kU/g protein | 7.3 |
CH2 | 3.5 | 10.8 | |
CH3 | 7.0 | 13.3 | |
SH1 | 3.0 | 7.5 | |
SH2 | 5.0 | 10.9 | |
SH3 | 7.0 | 13.2 |
2.2. Effects of the Two Hydrolysates on Cell Cycle Distribution of hFOB1.19 Cells
2.3. Anti-Apoptosis of the Two Hydrolysates towards hFOB1.19 Cells
Modes | Apoptosis Inducers | Percentage of Apoptotic Cells (%) | ||
---|---|---|---|---|
Model Group | CH3 Group | SH3 Group | ||
Apoptotic prevention | EP | 31.6 | 22.6 | 15.1 |
NaF | 19.5 | 17.7 | 12.4 | |
Apoptotic reversal | EP | 13.3 | 13.3 | 11.7 |
NaF | 14.5 | 15.3 | 11.0 |
3. Materials and Methods
3.1. Materials and Reagents
3.2. Preparation of the Hydrolysates
3.3. Chemical Analyses
3.4. Cell Culture Conditions and Cell Viability Assaying
3.5. Cell Cycle and Apoptosis Assaying
3.6. Statistical Analyses
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Pan, X.-W.; Zhao, X.-H. In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates towards Osteoblastic Cells (hFOB1.19). Int. J. Mol. Sci. 2015, 16, 13908-13920. https://doi.org/10.3390/ijms160613908
Pan X-W, Zhao X-H. In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates towards Osteoblastic Cells (hFOB1.19). International Journal of Molecular Sciences. 2015; 16(6):13908-13920. https://doi.org/10.3390/ijms160613908
Chicago/Turabian StylePan, Xiao-Wen, and Xin-Huai Zhao. 2015. "In Vitro Proliferation and Anti-Apoptosis of the Papain-Generated Casein and Soy Protein Hydrolysates towards Osteoblastic Cells (hFOB1.19)" International Journal of Molecular Sciences 16, no. 6: 13908-13920. https://doi.org/10.3390/ijms160613908