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Open AccessArticle

Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells

Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany
Department of Obstetrics and Gynecology, Ludwig Maximilians University of Munich, Maistraße 11, Munich 80337, Germany
Author to whom correspondence should be addressed.
Academic Editor: Yuping Bao
Int. J. Mol. Sci. 2015, 16(11), 26280-26290;
Received: 31 August 2015 / Revised: 13 October 2015 / Accepted: 15 October 2015 / Published: 3 November 2015
(This article belongs to the Special Issue Developmental and Reproductive Toxicity of Iron Oxide Nanoparticles)
PDF [2147 KB, uploaded 3 November 2015]


Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEONLA-BSA), or with dextran (SEONDEX). Both micronuclei testing and the detection of γH2A.X revealed no genotoxic effects of SEONLA-BSA, SEONDEX or SEONLA. Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system. View Full-Text
Keywords: superparamagnetic iron oxide nanoparticles; protein corona; cancer therapy and diagnosis; reproductive health; granulosa cells superparamagnetic iron oxide nanoparticles; protein corona; cancer therapy and diagnosis; reproductive health; granulosa cells

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Pöttler, M.; Staicu, A.; Zaloga, J.; Unterweger, H.; Weigel, B.; Schreiber, E.; Hofmann, S.; Wiest, I.; Jeschke, U.; Alexiou, C.; Janko, C. Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells. Int. J. Mol. Sci. 2015, 16, 26280-26290.

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